Recombinant Human BDNF Protein
|brain-derived neurotrophic factor||
BDNF is a member of the nerve growth factor family of trophic factors. In the brain BDNF has a trophic action on retinal, cholinergic, and dopaminergic neurons, and in the peripheral nervous system it acts on both motor and sensory neurons. Some protein domains of BDNF are identical with those of NGF and another neurotrophic factor, designated NT-3 (neurotrophin-3). Polyclonal antibodies raised against murine NGF have been shown to cross-react with both NT-3 and BDNF. Recombinant Human BDNF produced in E.coli is a homodimer, non-glycosylated, polypeptide chain containing 2 x 119 amino acids and having a total molecular mass of 26,984 Dalton.
|The ED50, as calculated via the dose-dependent induction of rat C6 glioma cell proliferation, is 1ug/ml.|
|119 αα||27.0 kDa||P23560
Sterile Filtered white lyophilized (freeze-dried) powder.
Lyophilized with no additives.
Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Lyophilized product is very stable at -20°C. Reconstituted material should be aliquoted and frozen at -20°C. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.
Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.
brain-derived neurotrophic factor, neurotrophin, abrineurin, Abrineu
MHSDPARRGE LSVCDSISEW VTAADKKTAV DMSGGTVTVL EKVPVSKGQL KQYFYETKCN PMGYTKEGCR GIDKRHWNSQ CRTTQSYVRA LTMDSKKRIG WRFIRIDTSC VCTLTIKRGR
FOR RESEARCH USE ONLY.