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Recombinant Human CD40 Ligand Protein

   
CD40 Ligand  

CD40L or CD154 is a membrane glycoprotein and differentiation antigen expressed on the surface of T-cells. The CD40 ligand stimulates B-cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD40 ligand has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also co-stimulates proliferation of activated T-cells and this is accompanied by the production of IFN-gamma, TNF-alpha, and IL2. Recombinant Human CD40L produced in E. coli is a non-glycosylated polypeptide chain.

 

The activity is determined by the dose production of IL-8 by human PBMCs and is typically 5-10 ng/mL.

         
149 αα 16.3 kDa P29965
>98% E. coli -20oC


Physical Appearance

Sterile Filtered white lyophilized (freeze-dried) powder.

Formulation

CD40L is lyophilized from 10mM Sodium Phosphate pH=7.5.

Reconstitution

Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.

Stability

Lyophilized product is very stable at -20 degrees. Reconstituted material should be aliquoted and frozen at -20. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.

Protein content determined by

UV spectroscopy at 280 nm. Analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.

Endotoxin Level

Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.

Alternative Names

CD40 ligand;  IGM; TRAP; HIGM1; CD40L; tumor necrosis factor (ligand) superfamily, member 5 (hyper-IgM syndrome); TNFSF5;  IMD3; CD154; tumor necrosis factor (ligand) superfamily member 5; gp39; CD40 antigen ligand; hCD40L; T-B cell-activating molecule; TNF-related activation protein;    T-BAM; T-cell antigen Gp39; Tumor necrosis factor ligand superfamily member 5; CD40-L;  CD154 antigen  

AA Sequence

MQKGDQNPQI AAHVISEASS KTTSVLQWAE KGYYTMSNNL VTLENGKQLT VKRQGLYYIY AQVTFCSNRE ASSQAPFIAS LWLKSPGRFE RILLRAANTH SSAKPCGQQS IHLGGVFELQ PGASVFVNVT DPSQVSHGTG FTSFGLLKL

 

FOR RESEARCH USE ONLY