Recombinant Human CD40 Ligand Protein
Growth factors from Cell Guidance Systems are high purity suitable for the most demanding applications where biological activity is required.
CD40 ligand; IGM; TRAP; HIGM1; CD40L; tumor necrosis factor (ligand) superfamily, member 5 (hyper-IgM syndrome); TNFSF5; IMD3; CD154; tumor necrosis factor (ligand) superfamily member 5; gp39; CD40 antigen ligand; hCD40L; T-B cell-activating molecule; TNF-related activation protein; T-BAM; T-cell antigen Gp39; Tumor necrosis factor ligand superfamily member 5; CD40-L; CD154 antigen
CD40L or CD154 is a membrane glycoprotein and differentiation antigen expressed on the surface of T-cells. The CD40 ligand stimulates B-cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD40 ligand has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also costimulates proliferation of activated T-cells and this is accompanied by the production of IFN-gamma, TNF-alpha, and IL2. Recombinant Human CD40L produced in E.coli is a non-glycosylated, Polypeptide chain containing 149 amino acids and having a molecular mass of 16,308 Daltons.
Sterile Filtered white lyophilized (freeze-dried) powder.
CD40L is lyophilized from 10mM Sodium Phosphate pH=7.5.
Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg’s/ml, which can be further diluted into other aqueous solutions.
Lyophilized product is very stable at -20 degrees. Reconstituted material should be aliquoted and frozen at -20. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.
Purity determined by:
Greater than 98% by reducing and non-reducing SDS-PAGE gel.
Analysis by RP-HPLC.
Protein content determined by:
UV spectroscopy at 280 nm.
Analysis by RP-HPLC calibrated against a known standard.
Quantitation on SDS-PAGE against a known standard.
Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.
H-CD40L is fully biologically active when compared to standard. The ED50 as determined using T-47D cells is
MQKGDQNPQI AAHVISEASS KTTSVLQWAE KGYYTMSNNL VTLENGKQLT VKRQGLYYIY AQVTFCSNRE ASSQAPFIAS LWLKSPGRFE RILLRAANTH SSAKPCGQQS IHLGGVFELQ PGASVFVNVT DPSQVSHGTG FTSFGLLKL
FOR RESEARCH USE ONLY.