Recombinant Human FGF-21 Protein
Growth factors from Cell Guidance Systems are high purity suitable for the most demanding applications where biological activity is required.
fibroblast growth factor 21, FGF-21
Recombinant Human FGF-21 produced in E.coli is a single, non-glycosylated polypeptide chain containing 182 amino acids and having a molecular mass of 19.5 kDa.
Sterile Filtered white lyophilized (freeze-dried) powder.
Lyopohilized from 10 mM NaP, pH 7.5 + 100 mM NaCl.
Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Lyophilized product is very stable at -20°C. Reconstituted material should be aliquoted and frozen at -20°C. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.
Purity Approximately than 95% as determined by:
Reducing and non-reducing SDS-PAGE.
Protein Content determined by:
UV spectroscopy at 280 nm.
Quantitation on SDS-PAGE against a known standard.
Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.
MHPIPDSSPL LQFGGQVRQR YLYTDDAQQT EAHLEIREDG TVGGAADQSP ESLLQLKALK PGVIQILGVK TSRFLCQRPD GALYGSLHFD PEACSFRELL LEDGYNVYQS EAHGLPLHLP GNKSPHRDPA PRGPARFLPL PGLPPALPEP PGILAPQPPD VGSSDPLSMV GPSQGRSPSY AS
RESEARCH USE ONLY