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Recombinant Rat IFN-gamma Protein

   

Interferon Gamma

 

The major interferon is produced by mitogenically or antigenically stimulated lymphocytes. It is structurally different from type I interferon and its major activity is immunoregulation. It has been implicated in the expression of class II histocompatibility antigens in cells that do not normally produce them, leading to autoimmune disease. Interferon gamma is produced mainly by T-cells and natural killer cells activated by antigens, mitogens, or alloantigens. It is produced by lymphocytes expressing the surface antigens CD4 and CD8. The synthesis of IFN-gamma is induced, among other things, by Interleukin-2, FGF-basic, and EGF. Recombinant Rat IFN-gamma is a single, non-glycosylated, polypeptide chain. 

 

Recombinant Rat Interferon gamma is fully biologically active when compared to standard. The specific activity as determined in a viral challenge assay using EMC virus on L929, corresponding to an ED50 of 0.8 ng/ml.

         
135 αα 15.6 kDa P01581
>95% E. coli -20oC


Physical Appearance

Sterile filtered white lyophilized (freeze-dried) powder.

Formulation

Rat IFN-gamma was lyophilized from 0.5X PBS pH-7.2.

Reconstitution

Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.

Stability

Lyophilized product is very stable at -20°C. Reconstituted material should be aliquoted and frozen at -20°C. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.

Protein Content determined by

UV spectroscopy at 280 nm. Analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.

Endotoxin Level

Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.

Alternative Names

Immune interferon, IFG, IFI

AA Sequence

MQGTLIESLE SLKNYFNSSS MDAMEGKSLL LDIWRNWQKD GNTKILESQI ISFYLRLFEV LKDNQAISNN ISVIESHLIT NFFSNSKAKK DAFMSIAKFE VNNPQIQHKA VNELIRVIHQ LSPESSLRKR KRSRC

 

RESEARCH USE ONLY