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Recombinant Human IL-3 Protein

   
Interleukin 3   Interleukin-3 (IL-3) is a cytokine produced by activated T-cells and mast cells that is active on a broad range of hematopoietic cells and in the nervous system and appears to be important in several chronic inflammatory diseases. Recombinant Human IL-3 is a single, non-glycosylated polypeptide chain.    Human IL-3 is fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of TF-1 cells is less then 0.1 ng/ml.
         
133 αα 15 kDa P08700
>95% E. coli -20oC

 

 

 


Physical Appearance

Sterile Filtered white lyophilized (freeze-dried) powder.

Formulation

Lyopohilized with no additives.

Reconstitution

Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with 20 mM Acetic acid at a concentration of 0.1 – 0.5 mg’s/ml, which can be further diluted into other aqueous solutions.

Stability

Lyophilized product is very stable at -20 degrees. Reconstituted material should be aliquoted and frozen at -20.

Protein Content determined by

UV spectroscopy at 280 nm. Analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.

Endotoxin Level

Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.

Alternative Names

interleukin 3 (colony-stimulating factor, multiple), P-cell-stimulating factor, MCGF,  IL-3, interleukin-3, MULTI-CSF, multilineage-colony-stimulating factor, Multipotential colony-stimulating factor, mast-cell growth factor, Hematopoietic growth factor, Mast cell growth factor, P-cell stimulating factor

AA Sequence

APMTQTTSLK TSWVNCSNMI DEIITHLKQP PLPLLDFNNL NGEDQDILME NNLRRPNLEA FNRAVKSLQN ASAIESILKN LLPCLPLATA APTRHPIHIK DGDWNEFRRK LTFYLKTLEN AQAQQTTLSL AIF

RESEARCH USE ONLY