Recombinant Human IL-11
Induced proliferation of T11 cells for Human IL-11. Cell proliferation was measured to calculate the ED50, which is as expected less than 2.5 ng/ml.
Non-reducing (-) and reducing (+) conditions in a 4 - 20% Tris-Glycine gel stained with Coomassie Blue. 1 μg of protein was loaded in each lane. Human IL-11 has a predicted Mw of 19.3 kDa.
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PODS® Growth Factors
The product is currently not available as PODS®, please check back in the future.Description
Interleukin-11 (IL-11) is a member of the gp130 family of cytokines. IL-11 functions to promote hematopoietic stem cell proliferation and megakaryocyte differentiation. In non-hematopoietic cell populations, IL-11 stimulates acute-phase proteins, modulates the development of immunoglobulin-producing B cells, and regulates bone turnover. IL-11 binds the IL-11Rα receptor to activate JAK downstream signaling. Human IL-11 shows activity on mouse cells.Activity Assay
Human IL-11 is fully biologically active when compared to standard. The activity is determined by the ability to induce T11 cells proliferation and it is typically less than 2.5 ng/ml. This corresponds to an expected specific activity of 4 x 105 units/mg.AA Sequence
MPGPPPGPPR VSPDPRAELD STVLLTRSLL ADTRQLAAQL RDKFPADGDH NLDSLPTLAM SAGALGALQL PGVLTRLRAD LLSYLRHVQW LRRAGGSSLK TLEPELGTLQ ARLDRLLRRL QLLMSRLALP QPPPDPPAPP LAPPSSAWGG IRAAHAILGG LHLTLDWAVR GLLLLKTRLAlternative Names
Interleukin-11, interleukin 11, IL11, IL 11| Product Details | |
|---|---|
| Length | 179 aa |
| Molecular Weight | 19.3 kDa |
| Structure | Monomer |
| Source | E. coli |
| Accession Number | P20809 |
| Purity | ≥95% determined by reducing and non-reducing SDS-PAGE |
| Endotoxin Level | ≤1.00 EU/μg as measured by kinetic LAL |
| Formulation | Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA) |
| Reconstitution | Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile 10 mM HCI at 0.1 mg/ml, which can be further diluted into other aqueous solutions. |
| Stability and Storage |
12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed. |
