Recombinant Human IL-11 Protein
Growth factors from Cell Guidance Systems are high purity suitable for the most demanding applications where biological activity is required.
interleukin 11, interleukin-11, AGIF1, oprelvekin, IL-11, Oprelvekin, Adipogenesis inhibitory factor
Recombinant Human IL-11 produced in E.coli is a single, non-glycosylated polypeptide chain containing 179 amino acids and having a molecular mass of 19,256 Daltons.
Sterile Filtered white lyophilized (freeze-dried) powder.
Lyopohilized with no additives.
Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Lyophilized product is very stable at -20°C. Reconstituted material should be aliquoted and frozen at -20°C. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.
Purity Greater than 95% as determined by:
Analysis by RP-HPLC.
Reducing and non-reducing SDS-PAGE.
Protein Content determined by:
UV spectroscopy at 280 nm.
Analysis by RP-HPLC calibrated against a known standard.
Quantitation on SDS-PAGE against a known standard.
Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.
Human IL-11 is fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of the proliferation of T11 cells was found to be less than 2 ng/ml.
MPGPPPGPPR VSPDPRAELD STVLLTRSLL ADTRQLAAQL RDKFPADGDH NLDSLPTLAM SAGALGALQL PGVLTRLRAD LLSYLRHVQW LRRAGGSSLK TLEPELGTLQ ARLDRLLRRL QLLMSRLALP QPPPDPPAPP LAPPSSAWGG IRAAHAILGG LHLTLDWAVR GLLLLKTRL
FOR RESEARCH USE ONLY.