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Recombinant Human IL-11 Protein

   
Interleukin 11   Interleukin 11 (IL-11) is a pleotropic cytokine closely related to IL-6 in function, but is overall one of the lesser characterized interleukins. In hematopoietic cell populations, IL-11 is thought to inducemegakaryocyte differentiation. In non-hematopoietic populations, IL-11 is thought to be able to stimulate hepatic acute-phase proteins. Human IL-11 can stimulate murine cells.Recombinant Human IL-11 is a single, non-glycosylated polypeptide chain.    Human IL-11 is fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of the proliferation of T11 cells was found to be less than 2 ng/ml.
         
179 αα 19.3 kDa P20809.1
>95% E. coli -20oC


Physical Appearance

Sterile Filtered white lyophilized (freeze-dried) powder.

Formulation

Lyopohilized with no additives.

Reconstitution

Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.

Stability

Lyophilized product is very stable at -20°C. Reconstituted material should be aliquoted and frozen at -20°C.  It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.

Protein Content determined by

UV spectroscopy at 280 nm. Analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.

Endotoxin Level

Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.

Alternative Names

interleukin 11, interleukin-11, AGIF1, oprelvekin, IL-11, Oprelvekin, Adipogenesis inhibitory factor

AA Sequence

MPGPPPGPPR VSPDPRAELD STVLLTRSLL ADTRQLAAQL RDKFPADGDH NLDSLPTLAM SAGALGALQL PGVLTRLRAD LLSYLRHVQW LRRAGGSSLK TLEPELGTLQ ARLDRLLRRL QLLMSRLALP QPPPDPPAPP LAPPSSAWGG IRAAHAILGG LHLTLDWAVR GLLLLKTRL

FOR RESEARCH USE ONLY