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Recombinant Mouse IL-27/p28 Protein

   
Interleukin 27 / p28   Recombinant Mouse IL-27/p28 Subunit, also known as Interleukin-30, is a member of the IL-12 family of cytokines. When combined with EBI3 (Epstein-Barr virus induced gene 3), the heterodimer formed is IL-27.  Mouse p28 is a proinflammatory cytokine inducing immunomodulatory effects. Current research is underway to delineate specific biological functions. Recombinant mouse p28 is a single-chain peptide.   Mouse p28 biological activity was measured via dose-dependent inhibition of TGF-beta & IL-6-induced IL-17A expression in mouse CD4 splenocytes.  50ng/ml of mouse p28 is capable of inhibiting >25% of IL-17A expression in this assay. 
         
207 αα 23.7 kDa Q8K3I6
>97% E. coli -20oC


Physical Appearance

Sterile Filtered white lyophilized (freeze-dried) powder.

Formulation

Lyophilized at a concentration of 1 mg/ml in 10 mM NaP, 7.5.

Reconstitution

Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 – 0.5 mg’s/ml, which can be further diluted into other aqueous solutions.

Stability

Lyophilized product is very stable at - 20 degrees. Reconstituted material should be aliquoted and frozen at -20. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.

Protein Content determined by

UV spectroscopy at 280 nm. Quantitation on SDS-PAGE against a known standard.

Endotoxin Level     

Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.

Alternative Names

interleukin 27,  IL30, p28, IL-27A, interleukin 30,  IL-27, IL-27 p28 subunit, IL-27-A    interleukin-27 subunit alpha, IL-27 subunit alpha, IL27p28, IL27-A, IL27A

AA Sequence

MFPTDPLSLQ ELRREFTVSL YLARKLLSEV QGYVHSFAES RLPGVNLDLL PLGYHLPNVS LTFQAWHHLS DSERLCFLAT TLRPFPAMLG GLGTQGTWTS SEREQLWAMR LDLRDLHRHL RFQVLAAGFK CSKEEEDKEE EEEEEEEEKK LPLGALGGPN QVSSQVSWPQ LLYTYQLLHS LELVLSRAVR DLLLLSLPRR PGSAWDS

RESEARCH USE ONLY