recombinant mouse IL27/p28 protein

Growth factors from Cell Guidance Systems are high purity suitable for the most demanding applications where biological activity is required. 

Aliases

interleukin 27,   IL30, p28,  IL-27A, interleukin 30,  IL-27, IL-27 p28 subunit, IL-27-A     interleukin-27 subunit alpha, IL-27 subunit alpha, IL27p28, IL27-A, IL27A

Description:

Recombinant Mouse IL-27/p28 Subunit, also known as Interleukin-30, is a member of the IL-12 family of cytokines.  When combined with EBI3 (Epstein-Barr virus induced gene 3), the heterodimer formed is IL-27.  Mouse p28 is a proinflammatory cytokine inducing immunomodulatory effects. Current research is underway to delineate specific biological functions.  Recombinant mouse p28 is a single-chain 207 amino acid peptide with a mass of approximately 23,697 Daltons.

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Physical Appearance:

Sterile Filtered white lyophilized (freeze-dried) powder.

Source:

E.coli

Formulation:

Lyophilized at a concentration of 1 mg/ml in 10 mM NaP, 7.5.

Reconstitution:

Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 – 0.5 mg’s/ml, which can be further diluted into other aqueous solutions.

Stability:

Lyophilized product is very stable at - 20 degrees. Reconstituted material should be aliquoted and frozen at -20. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.

Purity ~ 97% as determined by:

Reducing and non-reducing SDS-PAGE.

Protein Content determined by:

UV spectroscopy at 280 nm.

Quantitation on SDS-PAGE against a known standard.

Endotoxin Level:           

Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.

Biological Activity:

Mouse p28 biological activity was measured via dose-dependent inhibition of TGF-beta & IL-6-induced IL-17A expression in mouse CD4 splenocytes.  50ng/ml of mouse p28 is capable of inhibiting >25% of IL-17A expression in this assay. 

AA Sequence:

MFPTDPLSLQ ELRREFTVSL YLARKLLSEV QGYVHSFAES RLPGVNLDLL PLGYHLPNVS LTFQAWHHLS DSERLCFLAT TLRPFPAMLG GLGTQGTWTS SEREQLWAMR LDLRDLHRHL RFQVLAAGFK CSKEEEDKEE EEEEEEEEKK LPLGALGGPN QVSSQVSWPQ LLYTYQLLHS LELVLSRAVR DLLLLSLPRR PGSAWDS

RESEARCH USE ONLY