Recombinant Human IP-10 / CXCL10 Protein
chemokine (C-X-C motif) ligand 10, Small-inducible cytokine B10, IP-10,10 kDa, interferon gamma-induced protein, INP10, SCYB10, small inducible cytokine subfamily B (Cys-X-Cys) member 10, protein 10 from interferon (gamma)-induced cell line2, IFI101, C-X-C motif chemokine 10, crg-2, interferon-inducible cytokine IP-10, mob-1, gamma-IP10, gIP-10, gamma IP10, C7, Gamma-IP10
Among CXC chemokines, CXCL10 has been identified to play an important role in several endocrinological autoimmune diseases such as Hashimoto′s thyroiditis, Graves′disease and Type 1 diabetes mellitus.The chemokine IP- 10 (interferon-inducible protein of 10 kDa, CXCL10) is a chemoattractant for CXCR3+ T cells, binds to the G protein-coupled receptor CXCR3, which is found mainly on activated T cells and NK cells, and plays an important role in Th1-type inflammatory diseases. IP-10 also binds to glycosaminoglycans (GAGs), an interaction thought to be important for its sequestration on endothelial and other cells. Recombinant Human IP-10 produced in E.Coli is a single,non- glycosylated, polypeptide chain containing 77 amino acids and having a molecular mass of 8516 Dalton.
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Sterile Filtered white lyophilized (freeze-dried) powder.
Lyopohilized with no additives.
Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg’s/ml, which can be further diluted into other aqueous solutions.
Lyophilized product is very stable at -20 degrees. Reconstituted material should be aliquoted and frozen at -20. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.
Purity Greater than 97% as determined by:
Analysis by RP-HPLC.
Reducing and non-reducing SDS-PAGE.
Protein Content determined by:
UV spectroscopy at 280 nm.
Analysis by RP-HPLC calibrated against a known standard.
Quantitation on SDS-PAGE against a known standard.
Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.
Recombinant Human IP-10 is fully biologically active when compared to standard. The Biological activity is calculated by its ability to chemoattract human T lymphocytes at 5 – 40 ng/ml.
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