Recombinant Human IP-10 / CXCL10 Protein
|Interferon Inducible Protein 10 / CXCL10||
Among CXC chemokines, CXCL10 has been identified to play an important role in several endocrinological autoimmune diseases such as Hashimoto′s thyroiditis, Graves′ disease and Type 1 diabetes mellitus. IP-10 is a chemoattractant for CXCR3+ T cells, binds to the G protein-coupled receptor CXCR3, which is found mainly on activated T cells and NK cells, and plays an important role in Th1-type inflammatory diseases. IP-10 also binds to glycosaminoglycans (GAGs), an interaction thought to be important for its sequestration on endothelial and other cells. Recombinant human IP-10 is a single, non-glycosylated, polypeptide chain.
Recombinant human IP-10 is fully biologically active when compared to standard. The biological activity is calculated by its ability to chemoattract human T lymphocytes at 5 – 40 ng/ml.
|77 αα||8.5 kDa||P02778.2
Sterile filtered white lyophilized (freeze-dried) powder.
Lyophilized with no additives.
Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Lyophilized product is very stable at -20°C. Reconstituted material should be aliquoted and frozen at -20°C. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.
Protein Content determined by
UV spectroscopy at 280 nm. Analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.
Endotoxin level as measured by LAL is <0.01 ng/ug or <0.1 EU/ug.
IP10, chemokine (C-X-C motif) ligand 10, small-inducible cytokine B10, interferon gamma-induced protein, INP10, SCYB10, small inducible cytokine subfamily B (Cys-X-Cys) member 10, protein 10 from interferon (gamma)-induced cell line2, C-X-C motif chemokine 10, crg-2, interferon-inducible cytokine IP-10, gamma-IP10, gIP-10, gamma IP10
VPLSRTVRCT CISISNQPVN PRSLEKLEII PASQFCPRVE IIATMKKKGE KRCLNPESKA IKNLLKAVSK EMSKRSP
RESEARCH USE ONLY