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Recombinant Mouse MCP-3 / CCL7 Protein

   
Monocyte Chemotactic Protein 3   Mouse MCP-3, also known as CCL7 and SCYA7 is produced in a variety of tumor cells and has been shown to induce chemotaxis in monocytes, basophils, NK cells and dendrocytes.  Recombinant mouse MCP-3 is a non-glycosylated, polypeptide chain.   

Mouse MCP-3 is fully biologically active when compared to standard. The specific activity as determined by the ability of Mouse MCP-3 to chemoattract Balb/C mouse spleen MNCs at 10- 100ng/ml.

         
74 αα 8.5 kDa Q03366.1
>95% E. coli -20oC


Physical Appearance

Sterile Filtered white lyophilized (freeze-dried) powder.

Formulation

Lyopohilized with no additives.

Reconstitution

Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.

Stability

Lyophilized product is very stable at -20 degrees. Reconstituted material should be aliquoted and frozen at -20. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.

Endotoxin Level

Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.

Protein Content determined by

UV spectroscopy at 280 nm. Analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.

Alternative Names

chemokine (C-C motif) ligand 7, Monocyte chemoattractant protein 3, MCP3     Monocyte chemotactic protein 3, Small-inducible cytokine A7

AA Sequence

QPDGPNASTC CYVKKQKIPK RNLKSYRRIT SSRCPWEAVI FKTKKGMEVC AEAHQKWVEE AIAYLDMKTP TPKP

RESEARCH USE ONLY