recombinant mouse MCP-3 / CCL7 protein
Growth factors from Cell Guidance Systems are high purity suitable for the most demanding applications where biological activity is required.
Aliases
chemokine (C-C motif) ligand 7, Monocyte chemoattractant protein 3, MCP3 Monocyte chemotactic protein 3, Small-inducible cytokine A7
Description:
Mouse MCP-3, also known as CCL7 and SCYA7 is produced in a variety of tumor cells and has been shown to induce chemotaxis in monocytes, basophils, NK cells and dendrocytes. Recombinant mouse MCP-3 is produced in E.Coli,and is a non-glycosylated, Polypeptide chain containing 74 amino acids and having a molecular mass of 8510 Dalton.
Physical Appearance:
Sterile Filtered white lyophilized (freeze-dried) powder.
Source:
E.coli
Formulation:
Lyopohilized with no additives.
Reconstitution:
Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg’s/ml, which can be further diluted into other aqueous solutions.
Stability:
Lyophilized product is very stable at -20 degrees. Reconstituted material should be aliquoted and frozen at -20. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.
Purity Greater than 98% as determined by:
Analysis by RP-HPLC.
Reducing and non-reducing SDS- PAGE.
Endotoxin Level:
Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.
Protein Content determined by:
UV spectroscopy at 280 nm.
Analysis by RP-HPLC calibrated against a known standard.
Quantitation on SDS-PAGE against a known standard.
Biological Activity:
Mouse MCP-3 is fully biologically active when compared to standard. The specific activity as determined by the ability of Mouse MCP-3 to chemoattract Balb/C mouse spleen MNCs at 10- 100ng/ml.
AA Sequence:
QPDGPNASTC CYVKKQKIPK RNLKSYRRIT SSRCPWEAVI FKTKKGMEVC AEAHQKWVEE AIAYLDMKTP TPKP
