Recombinant Human SHH protein
Growth factors from Cell Guidance Systems are high purity suitable for the most demanding applications where biological activity is required.
Aliases
Sonic Hedgehog, Shh, HGG1
Description:
Recombinant Human Sonic Hedgehog is a member of a small group of secreted proteins that are essential for development in both vertebrates and invertebrates. Three mammalian hedgehog genes (sonic, desert, Indian) share about 60% homology. The Cys at position 25 has been substituted with Ile. Human SHH is a non-glycosylated polypeptide consisting of 175 amino acids with a molecular weight of 19,697 Da.
Physical Appearance:
Sterile Filtered white lyophilized (freeze-dried) powder.
Source:
E.coli
Formulation:
Lyophilized from 50 mM KP, 300 mM KCl, pH 7.5.
Reconstitution:
Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1mg/ml, which can be further diluted into other aqueous solutions.
Stability:
Lyophilized product is very stable at -20°C. Reconstituted material should be aliquoted and frozen at -20°C. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.
Purity Greater than 97% as determined by:
Reducing and non-reducing SDS-PAGE.
Analytical HPLC.
Endotoxin Level:
Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.
Protein Content determined by:
UV spectroscopy at 280 nm.
Quantitation on SDS-PAGE against a known standard.
Bioactivity:
Human recombinant Sonic Hedgehog is biologically active as compared to a standard. The ED50, measured by the dose-dependent induction of alkaline phosphatase production by C3H/10T1/2 fibroblasts, is 750ng/ml.
AA Sequence:
IIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKISRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRALDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSV AAKSGG
