Recombinant Human Activin A Protein
Growth factors from Cell Guidance Systems are high purity suitable for the most demanding applications where biological activity is required.
Alternative Names
EDF (erythroid differentiation factor); FRP (Follicle stimulating hormone releasing protein)
Description
Human Activin-A is a 26 kDa disulfide-linked homodimer of two beta A chains, each containing 116 amino acid residues. Activin-A is a TGF-beta family member with a wide range of biological activities including regulation of cellular proliferation and differentiation, and promotion of neuronal survival. The biological activities of Activin-A can be counteracted by inhibins and also by the diffusible TGF-beta antagonist, Follistatin.
Independent Assay
In addition to our assay, the activity of this producut has been independently verified in the lab of Dr Josué MFOPOU KUNJOM (VUB - Diabetes Research Centre) - by analysis of expression of CXCR4 in definitive endoderm
Physical Appearance
Sterile Filtered white lyophilized (freeze-dried) powder.
Source
E.coli
Stability
Lyophilized product is very stable at -20°C. Reconstituted material should be aliquoted and frozen at -20°C. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage
Reconstitution
Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1mg/ml, which can be further diluted into other aqueous solutions.
Formulation
Activin A was lyophilized from a volatile solution after reverse phase chromatography
Protein Content Determined by
UV spectroscopy at 280 nm.Analysis by RP-HPLC calibrated against a known standard.Quantitation on SDS-PAGE against a known standard
Analysis
by RP-HPLC.Reducing and non-reducing SDS-PAGE
Purity
>95% as detemined by gel electrophoresis
Endotoxin
Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.
Biological Activity
ED50 is 0.3 - 1.8 ng/ml h-Activin A using MPC-11 cells
