Recombinant Human Activin A Protein
Human Activin A is a disulfide-linked homodimer of two beta A chains. Activin A is a TGF-beta family member with a wide range of biological activities including regulation of cellular proliferation, differentiation and promotion of neuronal survival. The biological activity of Activin A can be counteracted by inhibins and also by the diffusible TGF-beta antagonist, Follistatin.
ED50 is 1.6 - 3.1 ng/ml human Activin A using MPC-11 cells. In addition to our standard assay, the activity of this product has been verified in the lab of Dr Josué Mfopou Kunjom (VUB - Diabetes Research Centre) - by analysis of CXCR4 in definitive endoderm.
|110 αα||26 kDa||P08476
“We rely on Cell Guidance Systems’ Activin A. Over the years, they have provided consistent quality at a competitive price, backed by excellent customer service”.
Jennifer Shay, Lab Manager for Drs. Chad Cowan and Kiran Musunuru, Harvard University
McCracken, K.W., et al., 2014. Nature.
Sterile filtered white lyophilized (freeze-dried) powder.
Lyophilized product is very stable at -20°C. Reconstituted material should be aliquoted and frozen at -20°C. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.
Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Activin A was lyophilized from a volatile solution after reverse phase chromatography.
Protein Content Determined by
UV spectroscopy at 280 nm. Analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.
Endotoxin level, as measured by LAL, is less than 0.01 ng/ug or 0.1 EU/ug.
EDF, erythroid differentiation factor, FRP, follicle stimulating hormone releasing protein
MGNICAKKQF FVSFKDIGWN DWIIAPSGYH ANYCEGECPS HIAGTSGSSL SFHSTVINHY RMRGHSPFAN LKSCCVPTKL RPMSMLYYDD GQNIIKKDIQ NMIVEECGCS