Recombinant Human NT-4
Induced proliferation of rat C6 cells assay for Human NT-4.
Non-reducing (-) and reducing (+) conditions in a 4 - 20% Tris-Glycine gel stained with Coomassie Blue. 1 μg of protein was loaded in each lane. Human NT-4 has a predicted Mw of 28.1 kDa (each monomer is 14 kDa).
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PODS® Growth Factors
This growth factor is also available as PODS® for sustained release, see here.Description
Neurotrophin-4 (NT-4) is an important member of the Nerve Growth Factor (NGF) family of proteins. Neurotrophins undergo paracrine and autocrine signaling to control neuronal survival, neuronal differentiation, and dendrite outgrowth. NT-4 is expressed ubiquitously and signals through the TrkB receptor tryrosine kinase.Activity Assay
Human NT-4 is fully biologically active when compared to standard. The activity is determined by the ability to induce rat C6 cells proliferation.AA Sequence
MGVSETAPAS RRGELAVCDA VSGWVTDRRT AVDLRGREVE VLGEVPAAGG SPLRQYFFET RCKADNAEEG GPGAGGGGCR GVDRRHWVSE CKAKQSYVRA LTADAQGRVG WRWIRIDTAC VCTLLSRTGR AAlternative Names
Migration Inhibitory Factor, GIF, phenylpyruvate tautomerase, glycosylation-inhibiting factor, L-dopachrome tautomerase| Product Details | |
|---|---|
| Length | 131 / 262 aa |
| Molecular Weight | 14.0 / 28.1 kDa |
| Structure | Dimer |
| Source | E. coli |
| Accession Number | P34130 |
| Purity | ≥95% determined by reducing and non-reducing SDS-PAGE |
| Endotoxin Level | ≤1.00 EU/μg as measured by kinetic LAL |
| Formulation | Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA) |
| Reconstitution | Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions. |
| Stability and Storage |
12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed. |
