Recombinant Human IL-27/EBI-3 Protein
|Interleukin-27 / EBI-3||
Epstein-Barr Virus Induced Gene-3 (EBI-3) is a secreted glycoprotein belonging to the hematopoietin receptor family and related to the p40 subunit of IL-12. It was identified by its induced expression in B-lymphocytes in response to Epstein-Barr virus infection. EBI-3 forms heterodimers with p28 to form IL-27 and with p35 to form IL-35. Both IL-27 and IL-35 have anti-inflammatory and regulatory activity. Recombinant Human EBI is a non-glycosylated polypeptide chain.
Assay data for human recombinant EBI-3 is based upon qualitative binding to anti-EBI-3 antibody.
|209 αα||23.3 kDa||Q14213||>90%||E. coli||-20°C|
Sterile filtered white lyophilized (freeze-dried) powder.
Lyopohilized with mannitol.
Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with 20 mM HCl at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Lyophilized product is very stable at -20°C. Reconstituted material should be aliquoted and frozen at -20°C. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.
Protein Content determined by
UV spectroscopy at 280 nm. Quantitation on SDS-PAGE against a known standard.
Endotoxin level as measured by LAL is
Epstein-Barr virus induced 3, IL-27 subunit beta, epstein-barr virus-induced gene 3 protein, interleukin-27 subunit beta, EBV-induced gene 3 protein, IL-27B, cytokine receptor, IL27B
RKGPPAALTL PRVQCRASRY PIAVDCSWTL PPAPNSTSPV SFIATYRLGM AARGHSWPCL QQTPTSTSCT ITDVQLFSMA PYVLNVTAVH PWGSSSSFVP FITEHIIKPD PPEGVRLSPL AERQLQVQWE PPGSWPFPEI FSLKYWIRYK RQGAARFHRV GPIEATSFIL RAVRPRARYY VQVAAQDLTD YGELSDWSLP ATATMSLGK
FOR RESEARCH USE ONLY