Recombinant Human GM-CSF Protein
Growth factors from Cell Guidance Systems are high purity suitable for the most demanding applications where biological activity is required.
Aliases
colony stimulating factor 2 (granulocyte-macrophage), GMCSF, granulocyte-macrophage colony stimulating factor, GM-CSF, Colony-stimulating factor sargramostim, CSF, Sargramostim, molgramostin, Molgramostin, granulocyte-macrophage colony-stimulating factor
Description:
Granulocyte Macrophage Colony Stimulating Factor is produced in response to a number of inflammatory mediators by mesenchymal cells present in the hemopoietic environment and at peripheral sites of inflammation. Granulocyte Macrophage-CSF is able to stimulate the production of neutrophilic granulocytes, macrophages, and mixed granulocyte-macrophage colonies from bone marrow cells and can stimulate the formation of eosinophil colonies from fetal liver progenitor cells. GM-CSF can also stimulate some functional activities in mature granulocytes and macrophages. GM-CSF receptors shows significant homologies with other receptors for hematopoietic growth factors , including IL2-beta, IL-3 , IL -6 , IL-7 , EPO and the Prolactin receptors. Recombinant Human Granulocyte Macrophage CSF produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 127 amino acids and having a molecular mass of 14,477 Daltons.
Physical Appearance:
Sterile Filtered white lyophilized (freeze-dried) powder.
Source:
E.coli
Formulation:
GM-CSF was lyophilized from 10 mM NaP, 7.5.
Reconstitution:
Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the portein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg’s/ml, which can be further diluted into other aqueous solutions.
Stability:
Lyophilized product is very stable at -20 degrees. Reconstituted material should be aliquoted and frozen at -20. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.
Purity Greater than 98% as determined by:
Analysis by RP-HPLC.
Reducing and non-reducing SDS-PAGE.
Protein Content determined by:
UV spectroscopy at 280 nm.
Analysis by RP-HPLC calibrated against a known standard.
Quantitation on SDS-PAGE against a known standard.
Endotoxin Level:
Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.
Biological Activity:
Human GM-CSF is fully biologically active when compared to standard. The ED50, as determined by the dose-dependent induction of human TF-1 cell proliferation, is 0.094 ng/ml.
AA Sequence:
MAPARSPSPS TQPWEHVNAI QEARRLLNLS RDTAAEMNET VEVISEMFDL QEPTCLQTRL ELYKQGLRGS LTKLKGPLTM MASHYKQHCP PTPETSCATQ IITFESFKEN LKDFLLVIPF DCWEPVQE
RESEACRH USE ONLY
