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Recombinant Human GM-CSF Protein

   
Granulocyte-Macrophage Colony-Stimulating Factor   Granulocyte Macrophage Colony Stimulating Factor is produced in response to a number of inflammatory mediators by mesenchymal cells present in the hemopoietic environment and at peripheral sites of inflammation. Granulocyte Macrophage-CSF is able to stimulate the production of neutrophilic granulocytes, macrophages, and mixed granulocyte-macrophage colonies from bone marrow cells and can stimulate the formation of eosinophil colonies from fetal liver progenitor cells. GM-CSF can also stimulate some functional activities in mature granulocytes and macrophages. GM-CSF receptors shows significant homologies with other receptors for hematopoietic growth factors, including IL2-beta, IL-3, IL -6, IL-7, EPO and the Prolactin receptors. Recombinant Human Granulocyte Macrophage CSF is a single, non-glycosylated polypeptide chain.    Human GM-CSF is fully biologically active when compared to standard. The ED50, as determined by the dose-dependent induction of human TF-1 cell proliferation, is 0.094 ng/ml.
         
127 αα 14.5 kDa P04141
>98% E. coli -20oC


Physical Appearance

Sterile filtered white lyophilized (freeze-dried) powder.

Formulation

GM-CSF was lyophilized from 10 mM NaP, 7.5.

Reconstitution

Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the portein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.

Stability

Lyophilized product is very stable at -20°C. Reconstituted material should be aliquoted and frozen at -20°C. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.

Protein Content determined by

UV spectroscopy at 280 nm. Analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.

Endotoxin Level

Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.

Alternative Names

colony stimulating factor 2 (granulocyte-macrophage), GMCSF, granulocyte-macrophage colony stimulating factor, Colony-stimulating factor, CSF, Sargramostim, molgramostin

AA Sequence

MAPARSPSPS TQPWEHVNAI QEARRLLNLS RDTAAEMNET VEVISEMFDL QEPTCLQTRL ELYKQGLRGS LTKLKGPLTM MASHYKQHCP PTPETSCATQ IITFESFKEN LKDFLLVIPF DCWEPVQE

 

RESEARCH USE ONLY