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Recombinant Human IL-6 Protein

   
 Interleukin-6  

 Interleukin-6 (IL-6) is an important proinflammatory and immunoregulatory cytokine expressed by various cells. Interleukin-6 has been shown to inhibit the growth of early stage and to promote the proliferation of advanced stage melanoma cells in vitro. 

 

Human IL-6 is fully biologically active when compared to standard. The ED50, as determined by the dose-dependant stimulation of mouse 7TD1 cells, is less then 0.08 ng/ml.

         
184 αα 21. 0 kDa P05231  >95% E. coli -20oC


Physical Appearance

Sterile filtered white lyophilized (freeze-dried) powder.

Formulation

Lyophilized from 10 mM Acetic acid.

Reconstitution

Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with 10 mM HCl at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.

Stability

Lyophilized product is very stable at -20°C. Reconstituted material should be aliquoted and frozen at -20°C. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.

Protein Content determined by

UV spectroscopy at 280 nm. Analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.

Endotoxin Level

Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.

Stem Cell Related Validation

Recombinant Interleukin 6 was shown to have excellent activity for the induction of human fibroblasts for use as feeder cells

Alternative Names

interleukin 6, interferon, beta 2, IFN-beta-2, CDF, HSF, B-cell stimulatory factor 2, HGF, BSF2, IFNB2, Interferon beta-2, B cell stimulatory factor-2, Hybridoma growth factor, B-cell differentiation factor, CTL differentiation factor, BSF-2, interleukin BSF-2

AA Sequence

PVPPGEDSKD VAAPHRQPLT SSERIDKQIR YILDGISALR KETCNKSNMC ESSKEALAEN NLNLPKMAEK DGCFQSGFNE ETCLVKIITG LLEFEVYLEY LQNRFESSEE QARAVQMSTK VLIQFLQKKA KNLDAITTPD PTTNASLLTK LQAQNQWLQD MTTHLILRSF KEFLQSSLRA LRQM

 

FOR RESEARCH ONLY