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Recombinant Human IL-6 Protein

   
 Interleukin-6  

 Interleukin-6 (IL-6) is an important proinflammatory and immunoregulatory cytokine expressed by T cells, macrophages and muscle cells. IL-6 signals through a receptor complex containing two receptors, IL-6Rα and gp130. IL-6 has an important function in promoting fever and can serve to stimulate an immune response to trauma. IL-6 is often used for growth of hybridoma cell lines. Human IL-6 is active on mouse and rat cells. Recombinant human IL-6 is a non-glycosylated protein.

 

Human IL-6 is fully biologically active when compared to standard. The ED50, as determined by the dose-dependant stimulation of mouse 7TD1 cells, is less than 1 ng/ml.

         
184 αα 21.0 kDa P05231  >95% E. coli -20°C


Physical Appearance

Sterile filtered white lyophilized (freeze-dried) powder.

Formulation

Lyophilized from 10 mM acetic acid.

Reconstitution

Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with 10 mM HCl at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.

Stability

Lyophilized product is very stable at -20°C. Reconstituted material should be aliquoted and frozen at -20°C. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.

Protein Content determined by

UV spectroscopy at 280 nm. Reducing and non-reducing SDS-PAGE.

Endotoxin Level

Endotoxin level as measured by LAL is <0.01 ng/ug or <0.1 EU/ug.

Stem Cell Related Validation

Recombinant IL-6 was shown to have excellent activity for the induction of human fibroblasts for use as feeder cells.

Alternative Names

Interleukin 6, interleukin-6, interferon beta 2, IFN-beta-2, IL6, CDF, HSF, B-cell stimulatory factor 2, HGF, BSF2, IFNB2, B cell stimulatory factor-2, hybridoma growth factor, B-cell differentiation factor, CTL differentiation factor, BSF-2, interleukin BSF-2

AA Sequence

PVPPGEDSKD VAAPHRQPLT SSERIDKQIR YILDGISALR KETCNKSNMC ESSKEALAEN NLNLPKMAEK DGCFQSGFNE ETCLVKIITG LLEFEVYLEY LQNRFESSEE QARAVQMSTK VLIQFLQKKA KNLDAITTPD PTTNASLLTK LQAQNQWLQD MTTHLILRSF KEFLQSSLRA LRQM

 

FOR RESEARCH ONLY