Recombinant Mouse IL-6
Induced proliferation of B9 cells for Mouse IL-6. Cell proliferation was measured to calculate the ED50, which is as expected less than 50 pg/ml.
Non-reducing (-) and reducing (+) conditions in a 4 - 20% Tris-Glycine gel stained with Coomassie Blue. 1 μg of protein was loaded in each lane. Mouse IL-6 has a predicted Mw of 21.9 kDa.
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PODS® Growth Factors
The product is currently not available as PODS®, please check back in the future.Description
Interleukin-6 (IL-6) is a cytokine that is expressed by T cells, macrophages, and muscle cells. IL-6 acts to stimulate an immune response upon infection or trauma. IL-6 has both pro-inflammatory and anti-inflammatory functions and signals through the cell-surface type 1 cytokine receptor complex containing the IL-6Ra and gp130 chains. Human IL-6 is active on mouse and rat cells.Activity Assay
Mouse IL-6 is fully biologically active when compared to standard. The activity is determined by the ability to induce B9 cells proliferation and it is typically less than 50 pg/ml. This corresponds to an expected specific activity of 2 x 107 units/mg.AA Sequence
MPVPPGEDSK DVAAPHRQPL TSSERIDKQI RYILDGISAL RKETCNKSNM CESSKEALAE NNLNLPKMAE KDGCFQSGFN EETCLVKIIT GLLEFEVYLE YLQNRFESSE EQARAVQMST KVLIQFLQKK AKNLDAITTP DPTTNASLLT KLQAQNQWLQ DMTTHLILRS FKEFLQSSLR ALRQMAlternative Names
Interleukin-6, interleukin 6, IL6, IL 6, IFN-β2, interferon β 2, B-cell differentiation factor, BSF-2, HSF, MGI-2, CTL differentiation factor| Product Details | |
|---|---|
| Length | 185 aa |
| Molecular Weight | 21 kDa |
| Structure | Monomer |
| Source | E. coli |
| Accession Number | P05231 |
| Purity | ≥95% determined by reducing and non-reducing SDS-PAGE |
| Endotoxin Level | ≤1.00 EU/μg as measured by kinetic LAL |
| Formulation | Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA) |
| Reconstitution | Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile 10 mM HCI at 0.1 mg/ml, which can be further diluted into other aqueous solutions. |
| Stability and Storage |
12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed. |
