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Recombinant Mouse IL-6 Protein

   
Interleukin-6   Interleukin-6 (IL-6), also know as BCDG, BCGF and BSF-2, is an important proinflammatory and immunoregulatory cytokine expressed by various cells. Interleukin-6 has been shown to inhibit the growth of early stage and to promote the proliferation of advanced stage melanoma cells in vitro. Recombinant Mouse IL-6 is a single, non-glycosylated polypeptide chain.    Recombinant mouse IL-6 is fully biologically active when compared to standards. The ED50, as determined by dose-dependent induction of 7TD1 cell proliferation, is 0.18 ng/ml.
         
187 αα 21.7 kDa P08505
>95% E. coli -20oC


Physical Appearance

Sterile filtered white lyophilized (freeze-dried) powder.

Formulation

The protein was lyophilized with no additives.

Reconstitution

Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized r-M-IL-6 in 10 mM HCl to 0.1-1.0 mg/mL to regain full activity, and can then be further diluted to other aqueous solutions.

Stability

Lyophilized product is very stable at -20°C. Reconstituted material should be aliquoted and frozen at -20°C. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.

Endotoxin Level

Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.

Protein Content determined by

UV spectroscopy at 280 nm. Analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.

Alternative Names

interleukin 6 (interferon, beta), IFN-beta-2, CDF, HSF, B-cell stimulatory factor 2, HGF, BSF2, IFNB2, Interferon beta-2, B cell stimulatory factor-2, hybridoma growth factor, B-cell differentiation factor, CTL differentiation factor, BSF-2, interleukin BSF-2

AA Sequence

MFPTSQVRRG DFTEDTTPNR PVYTTSQVGG LITHVLWEIV EMRKELCNGN SDCMNNDDAL AENNLKLPEI QRNDGCYQTG YNQEICLLKI SSGLLEYHSY LEYMKNNLKD NKKDKARVLQ RDTETLIHIF NQEVKDLHKI VLPTPISNAL LTDKLESQKE WLRTKTIQFI LKSLEEFLKV TLRSTRQT

 

RESEARCH USE ONLY