recombinant mouse IL6 protein
Growth factors from Cell Guidance Systems are high purity suitable for the most demanding applications where biological activity is required.
Aliases
interleukin 6 (interferon, beta), IFN-beta-2, IL-6, CDF, HSF, B-cell stimulatory factor 2, HGF, BSF2, interleukin-6, IFNB2, Interferon beta-2, B cell stimulatory factor-2, Hybridoma growth factor, B-cell differentiation factor, CTL differentiation factor, BSF-2, interleukin BSF-2
Description:
Interleukin (IL)-6, also know as BCDG, BCGF and BSF-2, is an important proinflammatory and immunoregulatory cytokine expressed by various cells. Interleukin-6 has been shown to inhibit the growth of early stage and to promote the proliferation of advanced stage melanoma cells in vitro. Recombinant Mouse IL-6 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 187 amino acids and having a molecular mass of 21,709 Daltons.
Physical Appearance:
Sterile Filtered white lyophilized (freeze-dried) powder.
Source:
E.coli
Formulation:
The protein was lyophilized with no additives.
Reconstitution:
Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized r-M-IL-6 in 10 mM HCl to 0.1-1.0 mg/mL to regain full activity, and can then be further diluted to other aqueous solutions.
Stability:
Lyophilized product is very stable at -20 degrees. Reconstituted material should be aliquoted and frozen at -20. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.
Purity Greater than 95% as determined by:
Reducing and non-reducing SDS-PAGE.
Endotoxin Level:
Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.
Protein Content determined by:
UV spectroscopy at 280 nm.
Analysis by RP-HPLC calibrated against a known standard.
Quantitation on SDS-PAGE against a known standard.
Biological Activity:
Recombinant mouse IL-6 is fully biologically active when compared to standards. The ED50, as determined by dose-dependent induction of 7TD1 cell proliferation, is .018 ng/ml.
AA Sequence:
MFPTSQVRRG DFTEDTTPNR PVYTTSQVGG LITHVLWEIV EMRKELCNGN SDCMNNDDAL AENNLKLPEI QRNDGCYQTG YNQEICLLKI SSGLLEYHSY LEYMKNNLKD NKKDKARVLQ RDTETLIHIF NQEVKDLHKI VLPTPISNAL LTDKLESQKE WLRTKTIQFI LKSLEEFLKV TLRSTRQT
