Recombinant Mouse MCSF Protein
Growth factors from Cell Guidance Systems are high purity suitable for the most demanding applications where biological activity is required.
Macrophage colony stimulating factor (M-CSF)
Macrophage colony stimulating factor (M-CSF) is hematopoietic growth factor produced by a wide variety of cells. M-CSF is known to stimulate differentiation of hematopoietic stem cells to monocyte-macrophage cell populations in culture. M-CSF acts through the CSF receptor 1. Although human M-CSF shows activity on mouse cells, mouse CSF shows no activity on human cells. Recombinant mouse M-CSF is a disulfide-linked homodimer, comprised of two 159 amino acid chains, with a total molecular weight of 36.8 kDa.
Sterile Filtered white lyophilized (freeze-dried) powder.
Lyophilized from 0.5 x PBS, pH 8.0.
Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg’s/ml, which can be further diluted into other aqueous solutions.
Lyophilized product is very stable at -20 degrees. Reconstituted material should be aliquoted and frozen at -20. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.
Purity Greater than 98% as determined by:
Analysis by RP-HPLC.
Reducing and non- reducing SDS-PAGE.
Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.
Protein Content determined by:
UV spectroscopy at 280 nm.
Analysis by RP-HPLC calibrated against a known standard.
Quantitation on SDS-PAGE against a known standard.
Recombinant mouse M-CSF is fully biologically active when compared to standards. The ED50, as determined by dose-dependent induction of M-NFS-60 cell proliferation, is 0.91 ng/ml.
MKEVSEHCSH MIGNGHLKVL QQLIDSQMET SCQIAFEFVD QEQLDDPVCY LKKAFFLVQD IIDETMRFKD NTPNANATER LQELSNNLNS CFTKDYEEQN KACVRTFHET PLQLLEKIKN FFNETKNLLE KDWNIFTKNC NNSFAKCSSR DVVTKP