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Recombinant Mouse MCSF Protein

   

Macrophage Colony Stimulating Factor

 

Macrophage colony stimulating factor (MCSF) is hematopoietic growth factor produced by a wide variety of cells. MCSF is known to stimulate differentiation of hematopoietic stem cells to monocyte-macrophage cell populations in culture. MCSF acts through the CSF receptor 1. Although human MCSF shows activity on mouse cells, mouse MCSF shows no activity on human cells. Recombinant mouse MCSF is a disulfide-linked homodimer.

 

Recombinant mouse MCSF is fully biologically active when compared to standards. The ED50, as determined by dose-dependent induction of M-NFS-60 cell proliferation, is 0.91 ng/ml.

         
156 αα 36.8 kDa P07141
>98% E. coli -20oC

 

Product Citation

Hv1 proton channels differentially regulate the pH of neutrophil and macrophage phagosomes by sustaining the production of phagosomal ROS that inhibit the delivery of vacuolar ATPases. El Chemaly, A., et al. 2014. J Leukoc Biol.


Physical Appearance

Sterile filtered white lyophilized (freeze-dried) powder.

Formulation

Lyophilized from 0.5 x PBS, pH 8.0.

Reconstitution

Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.

Stability

Lyophilized product is very stable at -20°C. Reconstituted material should be aliquoted and frozen at -20°C. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.

Endotoxin Level

Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.

Protein Content determined by

UV spectroscopy at 280 nm. Analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.

Alternative Names

Macrophage colony stimulating factor, M-CSF

AA Sequence

MKEVSEHCSH MIGNGHLKVL QQLIDSQMET SCQIAFEFVD QEQLDDPVCY LKKAFFLVQD IIDETMRFKD NTPNANATER LQELSNNLNS CFTKDYEEQN KACVRTFHET PLQLLEKIKN FFNETKNLLE KDWNIFTKNC NNSFAKCSSR DVVTKP

 

RESEARCH USE ONLY