Recombinant Mouse LIF
Expression of the two common pluripotency markers Nanog and Oct-4 in the presence of mouse LIF was assessed with RT-PCR. Gapdh is the loading control.
Non-reducing (-) and reducing (+) conditions in a 4 - 20% Tris-Glycine gel stained with Coomassie Blue. 1 μg of protein was loaded in each lane. Human MCP-1 has a predicted Mw of 8.7 kDa.
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PODS® Growth Factors
The product is currently not available as PODS®, please check back in the future.Description
Leukemia Inhibitory Factor (LIF) is a member of the interleukin-6 (IL-6) family that is made by a variety of adult and embryonic tissues. LIF signals through the glycoprotein 130 (gp130)/LIF receptor (LIFR) heterodimer to activate STAT3 and MAPK signaling. LIF functions during hematopoietic differentiation, neuronal cell differentiation, kidney development, and inflammatory processes. The application of mouse LIF to long-term culture systems promotes mouse embryonic stem cell (ESC) self-renewal and pluripotency, similar to the functional activity of FGF-2 in human ESC cell culture systems.Activity Assay
Mouse LIF is fully biologically active when compared to standard. The specific activity of the sample is approximately 1 x 108 units/mg, in which 106 units are identical of 10 µg of pure protein sufficient to treat 1.0 L of stem cells including human embryonic stem cells, neural stem cells, hematopoietic stem cells, mesenchymal stem cells and induced pluripotent stem cells.AA Sequence
MSPLPITPVN ATCAIRHPCH GNLMNQIKNQ LAQLNGSANA LFISYYTAQG EPFPNNVEKL CAPNMTDFPS FHGNGTEKTK LVELYRMVAY LSASLTNITR DQKVLNPTAV SLQVKLNATI DVMRGLLSNV LCRLCNKYRV GHVDVPPVPD HSDKEAFQRK KLGCQLLGTY KQVISVVVQA FAlternative Names
Leukocyte Inhibitory Factor, leukemia inhibitory factor, cholinergic differentiation factor| Product Details | |
|---|---|
| Length | 181 aa |
| Molecular Weight | 20 kDa |
| Structure | Monomer |
| Source | E. coli |
| Accession Number | P09056 |
| Purity | ≥95% determined by reducing and non-reducing SDS-PAGE |
| Endotoxin Level | ≤1.00 EU/μg as measured by kinetic LAL |
| Formulation | Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA) |
| Reconstitution | Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile 10 mM acetic acid at 0.1 mg/ml, which can be further diluted into other aqueous solutions. |
| Stability and Storage |
12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed. |
Citations
Russell R, Ilg M, Lin Q, Wu G, Lechel A, Bergmann W, Eiseler T, Linta L, Kumar PP, Klingenstein M. A Dynamic Role of TBX3 in the Pluripotency Circuitry. (2015). Stem Cell Research 5(6)
