Exo-spin™ Exosome Purification Kit
Exo-spin™ generates superior yields and higher quality whole exosomes compared with any available alternative. No ultracentrifugation is required. No organic buffers are used and the precipitant is purified away.
The Exo-spin™ kit is suitable for the preparation of pure, functional exosomes from a variety of biological fluids including cell culture media, urine and saliva. For blood plasma and sera, Exo-spin™ blood (Cat EX02) is available.
Exosomes purified using Exo-spin™ are suitable for a variety of downstream applications including RNA analysis, protein analysis and funtional use.
For large sample volumes, often used with cell cultue media and urine extraction, for example, EX01-25L offers unsurpassed economy.
Simple, easy-to-follow two hour protocol.
(1) Remove cells and cellular debris
(2) Pellet microvesicle containing fraction
(3) Purify exosomes.
NanoSight analysis of samples prepared from cell culture medium
(a) Exo-spin™ yields significantly higher numbers of vesicles of the expected 40-120 nm size range than alternatives including
(c) competitor kit
The size distribution profile obtained with Exo-spin™ most closely resembles ultra-centrifugation.
Transmission electron microscope analysis
Shows Exosomes purified using Exo-spin™ have expected morphology and size range.
Lower contaminating rRNA levels
Analysis on Bioanalyzer instrument shows RNA preps (Trizol) following a) Exo-spin™ purification from cell culture samples compared with b) ultracentrifugation or c) competitor kit.
Exo-spin™ columns should be spun at 50xG. You may calculate the Gforce generated by your centrifuge using this calculator. Alternatively (or cross check), use our Nomograph.