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Exo-spin™ Exosome Purification Kit

 Exospin size exclusion columns  

Exo-spin™ generates superior yields and higher quality whole exosomes. No ultracentrifugation is required. No organic buffers are used and the precipitant is purified away.

The Exo-spin™ kit is suitable for the preparation of pure, functional exosomes from a variety of biological fluids including cell culture media, urine and saliva. For blood plasma and sera, Exo-spin™ blood (Cat EX02) is available.

Exosomes purified using Exo-spin™ are suitable for a variety of downstream applications including RNA analysis, protein analysis and funtional use.

For large sample volumes, often used with cell culture media and urine extraction, EX01-25L offers unsurpassed economy.

exospin kit

exosome purification reference  

Lane et al perform an independent analysis of various exosome purification technologies, including Exo-spin™, using a model liposome system.  

Analysis of exosome purification methods using a model liposome system and tunable-resistive pulse sensing. Rebecca E. Lane,Darren Korbie,Will Anderson,Ramanathan Vaidyanathan& Matt Trau. Scientific Reports 5, Article number: 7639 doi:10.1038/srep07639


Simple protocol TEM photograph

Simple, easy-to-follow two hour protocol. 

(1) Remove cells and cellular debris. (2) Pellet microvesicle containing fraction.(3) Purify exosomes

Transmission electron microscope analysis

Shows Exosomes purified using Exo-spin™ have expected morphology and size range.


Nanosight analysis Bioanalyzer analysis

NanoSight analysis of samples prepared from cell culture medium 

(a) Exo-spin™ yields significantly higher numbers of vesicles of the expected 40-120 nm size range than alternatives including (b) ultracentrifugation (c) competitor kit. The size distribution profile obtained with Exo-spin™ most closely resembles ultra-centrifugation. 

Lower contaminating rRNA levels

Analysis on Bioanalyzer instrument shows RNA preps (Trizol) following a) Exo-spin™ purification from cell culture samples compared with b) ultracentrifugation or c) competitor kit.



Exo-spin™ columns should be spun at 50xG. You may calculate the Gforce generated by using our Nomograph.

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Exo-spin™ Blood Exosome Purification Kit