Cell Guidance Systems offers a range of kits and products to assay, track and detect microvesicles.
TRIFic™ detection assay
The TRIFic™ exosome assay is similar to an ELISA assay, however, there are some significant differences. Unlike an ELISA assay, there is no enzymatic reaction. Rather, the target is detected directly with a Europium label. TRIFic™ exosome assays quickly provide quantitative data from purified or unpurified samples, including direct measurement of exosomes in plasma in a convenient 96-well format. TRIFic™ exosome assays are being made available for widely used markers of exosomes; the tetraspanin proteins CD9, CD63 and CD81. TRIFic™ exosome assays deliver clear, consistent data.
The simplicity of the assay results in a high degree of reproducibility.
Europium is used for detection, providing a high degree of sensitivity.
Only antigens displayed in multiple copies on a support within the sample (as with tetraspanins on exosomes) are detectable with the assay, resulting in increased specificity.
ExoFLARE™ tracking assay
ExoFLAREs™ utilize a combination of a FLARE (FLuorescence Activating Response Element) protein tag together with a pro-fluorophore dye. Neither the protein or dye fluoresce in isolation. When the protein binds to the dye, it causes a change in structure which results in fluorescence. The dye and protein form an unstable bond with a continuous turnover of the dye. This results in sustained fluorescence without the levels of photo-bleaching associated with fluorescent proteins. This allows ExoFLAREs™ to be monitored for extensive periods to allow tracking of movement of dyes.
Exosome antigen antibodies from Cell Guidance Systems detect some of the most widely studied exosome antigens. Our antibodies have been validated for the detection of exosomes. We offer the same antibodies used in our TRIFic™ assay kit.