aCGH services for human cells

Code Description Price Qty
K20 aCGH, single customer DNA sample, our control DNA £350.00
K21 aCGH, two DNA samples from customer £525.00

Service Description

Array CGH is performed on the Agilent 8x60K platform.  This platform utilizes two differentially labeled DNA samples, co-hybridized to an array with ~55,000 oligonucleotide probes simultaneously.

We provide two service options for aCGH:

  1. A single sample provided by the customer is labeled and hybridized against a control DNA sample provided by ourselves (Cat code K20)
  2. Two samples, both provided by the customer, are co-hybridized (Cat code K21)

Service time from receipt of samples is typically 15 days.

A report is emailed describing any abnormalities identified.

aCGH vs AGH

                 

Comparison of aCGH and AGH methods.

For aCGH, (a) the sample and a normal control are labelled with fluorophores which fluoresce at different wavelengths. (b) The relative intensities of these wavelengths are compared. (c) The probes are plotted against their chromosomal origin.  Duplication events are indicated by clusters of probes that show an increase in the relative intensity of test vs control fluorophore. Deletion events are re indicated by clusters of probes that show a decrease in the relative intensity of test vs control fluorophore.    

For AGH, (a) only the sample is labelled with a fluorophore. (b) The intensities of the signals are read and (c) compared in-silico to a database of thousands of normal and disease-specific cells (d) The probes are plotted against their chromosomal origin. (Duplication events are indicated by clusters of probes that show an increase in the relative intensity of test vs control samples database. Deletion events are re indicated by clusters of probes that show a decrease in the relative intensity of test vs control sample database.  

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