AGH service for human cells

Code Description Price Qty
K22 AGH £525.00

Service Description

Array Genome Hybridization (AGH) performed using Affymetrix CytoScan 750K platform

  • Hybridization data from a single test DNA is performed. The data generated by this hybridization is compared in-silico with a large database of >10,000 control hybridizations.
  • This assay is particularly useful for acquired loss of heterozygosity (LOH).
  • The assay is performed to clinical standards but is for non-clinical research use only.
  • The service typically takes 15 days to complete.
  • A report detailing any abnormalities identified is sent to the customer by email.

aCGH vs AGH

                 

Comparison of aCGH and AGH methods.

For aCGH, (a) the sample and a normal control are labelled with fluorophores which fluoresce at different wavelengths. (b) The relative intensities of these wavelengths are compared. (c) The probes are plotted against their chromosomal origin.  Duplication events are indicated by clusters of probes that show an increase in the relative intensity of test vs control fluorophore. Deletion events are re indicated by clusters of probes that show a decrease in the relative intensity of test vs control fluorophore.    

For AGH, (a) only the sample is labelled with a fluorophore. (b) The intensities of the signals are read and (c) compared in-silico to a database of thousands of normal and disease-specific cells (d) The probes are plotted against their chromosomal origin. (Duplication events are indicated by clusters of probes that show an increase in the relative intensity of test vs control samples database. Deletion events are re indicated by clusters of probes that show a decrease in the relative intensity of test vs control sample database.      

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