ETS-Embryo medium

Code Description Price Qty
M13-25 ETS-Embryo Medium, 25 ml (5 ml aliquots) £78.00
M13-100 ETS-Embryo Medium, 100 ml £148.00
ETS-Embryo medium packaging
ETS-Embryo structures

Overview

ETS-Embryo Medium supports the development of artificial embryo structures, known as ETS-embryos, when trophoblast stem cells and embryonic stem cells are co-cultured.

A 2017 landmark study of trophoblast and embryonic stem cells (ESCs) has produced a breakthrough for stem cell research. Prof Magdalena Zernicka-Goetz and her colleagues at Cambridge University found that simply growing these two cells together leads to the development of "artificial embryo" structures termed ETS-embryos (Embryonic Trophoblast Stem embryos). These ETS-embryos strongly resemble mouse embryos in the days immediately following their implantation into the womb. Key to this success was development of “ETS-Embryo Medium” to culture the two stem cell types.

The efficiency of the process is remarkable. Just under 20% of the clusters of cells identified in co-cultures of trophoblast and embryonic stem cells were ETS-embryos. This efficiency offers the prospect of new applications, beyond the scope of embryo research. ETS-embryos are set to transform stem cell science. Potential applications include improving the efficiency of generation of transgenic animals for research and novel ways to make tissues and organs for stem cell research.

ETS-Embryo Medium is available only from Cell Guidance Systems.

Attributes

  • Optimized for robust, efficient generation of ETS-embryos  
  • Simple, easy-to-follow protocol  
  • Functionally tested  
  • Available in convenient 25 ml (5 x 5 ml) or 100 ml pack sizes
  • Bulk quantities available

Prof Magdalena Zernicka-Goetz and Sarah Harrison talk about ETS embryos

 

Product Data

ETS artificial embryos

A series of images showing the development of ETS embryos over a period of five days. Trophoblast-derived cells are stained with a DNA-marker only (blue) and ES-derived cells stained with embryonic marker OCT4 (red)
ETS artificial embryosStaining of an ETS-Embryo with markers for DNA, OCT4, and E-cadherin combined to produce a composite 3D image

 

ETS artificial embryos

ESC cells expressing GFP and cherry histone used to highlight the embryonic cells in an ETS-embryo

 

ETS artificial embryos

ETS-embryos stained with markers of trophoblast cells (Eomes- blue) and ES cells (OCT4 - pink)

References

Sarah Ellys Harrison, Berna Sozen, Neophytos Christodoulou, Christos Kyprianou and Magdalena Zernicka-Goetz. Assembly of embryonic and extra-embryonic stem cells to mimic embryogenesis in vitro. (2017). Science 356(6334).

Product Documents

User Guide Brochure