Pluripro® medium

Code Description Price Qty
MK01 Pluripro medium, 500 ml £135.00
M04 Pluripro Matrix, 50 ml £48.00
Oct4 stained cells
Pluripro medium

Overview

Pluripro®  is trusted in leading labs in Europe, Asia, and North America. Maintaining cells in culture is a core activity of any stem cell lab. It is also one of the most time-consuming. Pluripro®  has been designed to significantly reduce the amount of time required to maintain cells. From the simple pour-on-pour-off preparation of the culture surface using Pluripro® matrix to the 15-minute passaging protocol (for two independent lines performed in parallel), everything about Pluripro® has been designed for ease of use. The robustness of the system produces uniformity that is critical for optimized differentiation.

The most effective way to culture human pluripotent cells

Pluripro®  is a fully defined, serum-free culture system that supports high purity, confluent expansion of human pluripotent cells without the use of feeder cells. The Pluripro® system is specifically designed to make stem cell culture routine and robust, reducing labor intensity and increasing consistency. The system is composed of a carefully matched matrix coating and fully defined medium which together provide an ideal environment for the growth and maintenance of human pluripotent cells. Pluripro®  generates robust cultures where pluripotency rates above 98% are routinely maintained. Pluripro® does not utilize FGF-2.

Product Data

 
Cells cultured in Pluripro® grow uniformly across the culture surface providing a highly homogeneous pluripotent population and an excellent, uniform starting material for directed differentiation. Oct4 staining shown. Oct4 staining shown.
Cells cultured in the Pluripro® culture system routinely demonstrate staining of pluripotency markers across >98% cells when subjected to FC analysis. Data shown is for SSEA-4.

 

Cells can be a colony or confluent cultured in Pluripro®. Normal karyotype is retained over an extended passage. Karyotype at passage 20 is shown. Stable karyotype has been maintained to passage 20 in 2/2 independent cultures in our labs.
Beta II tubulin staining of pluripotent cells
Pluripotent human cells grown in Pluripro® will readily differentiate when placed in the appropriate environment. Embryoid bodies spontaneously differentiate into cells expressing markers for each of the three germ layers. Beta III tubulin (ectoderm marker) staining shown above.
Pluripotent human cells grown in Pluripro® will readily differentiate when placed in the appropriate environment. Embryoid bodies spontaneously differentiate into cells expressing markers for each of the three germ layers. Smooth muscle actin (mesoderm marker).
Pluripotent human cells grown in Pluripro® will readily differentiate when placed in the appropriate environment. Embryoid bodies spontaneously differentiate into cells expressing markers for each of the three germ layers. FoxA2 (endoderm marker).

 

Citations

Iefremova V, Manikakis G, Krefft O, Jabali A, Weynans K, Wilkens R, Marsoner F, Brandl B, Muller FJ, Koch P, Ladewig J. An Organoid-Based Model of Cortical Development Identifies Non-Cell-Autonomous Defects in Wnt Signaling Contributing to Miller-Dieker Syndrome. (2017). Cell Reports 19(1): 50-59.

Faedo A, Laporta A, Segnali A, Galimberti M, Besusso D, Cesana E, Belloli S, Moresco RM, Tropiano M, Fuca E, et al. Differentiation of human telencephalic progenitor cells into MSNs by inducible expression of Gsx2 and Ebf1. (2017). PNAS 114(7): E1234-1242.

Takenobu Nii, Hiroshi Kohara, Tomotoshi Marumoto, Tetsushi Sakuma, Takashi Yamamoto, and Kenzaburo Tani. Single-Cell-State Culture of Human Pluripotent Stem Cells Increases Transfection Efficiency. (2016). BioResearch 5: 127-136.

Mertens J, Paquola AC, Ku M, Hatch E, Bohnke L, Ladjevardi S, McGrath S, Campbell B, Lee H, Herdy JR, et al. Directly Reprogrammed Human Neurons Retain Aging-Associated Transcriptomic Signatures and Reveal Age-Related Nucleocytoplasmic Defects. (2015). Cell Stem Cell 17(6): 705-718.

Nagano T, Varnai C, Schoenfelder S, Javierre BM, Wingett SW, Fraser P. Comparison of Hi-C results using in-solution versus in-nucleus ligation. (2015). Genome Biol 16: 175.

lessia Delli Carri, Marco Onorati, Valentina Castiglioni, Andrea Faedo, Stefano Camnasio, Mauro Toselli, Gerardo Biella, Elena Cattaneo. Human Pluripotent Stem Cell Differentiation into Authentic Striatal Projection Neurons. (2013). Stem Cell Reviews and Reports 9: 461-474.

Julia Ladewig, Philipp Koch, Oliver Brustle. Auto-attraction of neural precursors and their neuronal progeny impairs neuronal migration. (2013). Nature Neuroscience 17: 24–26.

 

Documents

User Guide MSDS