PODS™ Human BMP-4
PODS™ TechnologyPODS™ proteins are made using an insect cell expression system in which the active protein is co-expressed alongside polyhedrin carrier protein. Polyhedrin forms microcrystals within insect cells which specifically capture the active protein to form a co-crystal complex. The active protein is captured in its nascent, natively folded form with limited scope for proteolytic degradation. Consequently, excellent levels of bioactivity are observed. The PODS™ crystals provide a sustained release mechanism and can be used to functionalize surfaces. For further details, please refer to the PODS™ Technology page.
Product DescriptionThe product contains the polyhedrin protein co-crystalized with Human BMP-4. Bone Morphogenic Protein 4 (BMP-4) is a member of the Bone Morphogenetic Protein (BMP) family. These proteins are synthesized as large precursor molecules which are cleaved by proteolytic enzymes. BMP proteinsstimulate the production of bone matrix proteins and osteoclasts proliferation, and can be found on tissues related with bone or cartilage growth.
PODS™ crystals provide a depot of proteins which are steadily secreted. It has been estimated that the biological activity of 50 million PODS™ crystals generates the same peak dose as 3.3 μg of standard recombinant protein. However, at 5 days following the start of seeding the PODS™ crystals, there are more than 50% of these peak levels still present in the culture system. Ultimately, the amount of PODS™ crystals that is optimal for a particular experiment should be determined empirically. Based on previous data, we suggest using 50 million PODS™ crystals in place of 3.3 μg of standard growth factor as a starting point."
To control for cross-reactivity with cells or as a negative control, we recommend using PODS™ growth factors alongside PODS™ Empty crystals, as the latter do not contain or release cargo protein.
AA SequenceMADVAGTSNR DFRGREQRLF NSEQYNYNNS KNSRPSTSLY KKAGSIPGNR MLMVVLLCQV LLGGASHASL IPETGKKKVA EIQGHAGGRR SGQSHELLRD FEATLLQMFG LRRRPQPSKS AVIPDYMRDL YRLQSGEEEE EQIHSTGLEY PERPASRANT VRSFHHEEHL ENIPGTSENS AFRFLFNLSS IPENEAISSA ELRLFREQVD QGPDWERGFH RINIYEVMKP PAEVVPGHLI TRLLDTRLVH HNVTRWETFD VSPAVLRWTR EKQPNYGLAI EVTHLHQTRT HQGQHVRISR SLPQGSGNWA QLRPLLVTFG HDGRGHALTR RRRAKRSPKH HSQRARKKNK NCRRHSLYVD FSDVGWNDWI VAPPGYQAFY CHGDCPFPLA DHLNSTNHAI VQTLVNSVNS SIPKACCVPT ELSAISMLYL DEYDKVVLKN YQEMVVEGCG CR
Alternative NamesBone morphogenetic protein 4, bone morphogenetic protein 4A, BMP-4A, BMP4, BMP4A
|Molecular Weight||103 kDa|
|Source||Spodoptera frugiperda (Sf9) cell culture|
|Endotoxin Level||<0.06 EU/ml as measured by gel clot LAL assay|
|Formulation||PODS™ were lyophilized from a volatile solution|
PODS™ protein crystals may be reconstituted at 200 million PODS™/ml in water. 20% glucose has a buoyant density closer to PODS™ protein crystals and can be useful for aliquoting.
PODS™ protein crystals are highly stable when stored in aqueous solution (pH range 6 - 8).
|Stability and Storage||Upon receipt, store at 4°C. PODS™ protein crystals are stable for at least 1 year when dry and 6 months when resuspended.|