PODS™ Human Wnt-3a
DescriptionWnt-3a signalling has been implicated in the control of differentiation of stem cells. Wnt proteins have been shown to regulate cell-to-cell interactions during embryogenesis, more specifically regulating mesoderm differentiation and osteogenesis. The Wnts have also been shown to have putative roles in the regulation of adult stem cells.
PODS™ crystals provide a depot of proteins which are steadily secreted. It has been estimated that the biological activity of 50 million PODS™ crystals generates the same peak dose as 3.3 μg of standard recombinant protein. However, at 5 days following the start of seeding the PODS™ crystals, there are more than 50% of these peak levels still present in the culture system. Ultimately, the amount of PODS™ crystals that is optimal for a particular experiment should be determined empirically. Based on previous data, we suggest using 50 million PODS™ crystals in place of 3.3 μg of standard growth factor as a starting point."
To control for cross-reactivity with cells or as a negative control, we recommend using PODS™ growth factors alongside PODS™ Empty crystals, as the latter do not contain or release cargo protein.
AA SequenceMADVAGTSNR DFRGREQRLF NSEQYNYNNS KNSRPSTSLY KKAGFMAPLG YFLLLCSLKQ ALGSYPIWWS LAVGPQYSSL GSQPILCASI PGLVPKQLRF CRNYVEIMPS VAEGIKIGIQ ECQHQFRGRR WNCTTVHDSL AIFGPVLDKA TRESAFVHAI ASAGVAFAVT RSCAEGTAAI CGCSSRHQGS PGKGWKWGGC SEDIEFGGMV SREFADAREN RPDARSAMNR HNNEAGRQAI ASHMHLKCKC HGLSGSCEVK TCWWSQPDFR AIGDFLKDKY DSASEMVVEK HRESRGWVET LRPRYTYFKV PTERDLVYYE ASPNFCEPNP ETGSFGTRDR TCNVSSHGID GCDLLCCGRG HNARAERRRE KCRCVFHWCC YVSCQECTRV YDVHTCKNPG SRAGNSAHQP PHPQPPVRFH PPLRRAGKVP
Alternative NamesWingless type 3, MMTV integration site family member 3A, Wnt 3a, Wnt3a
|Molecular Weight||48 kDa|
|Source||Spodoptera frugiperda (Sf9) cell culture|
|Endotoxin Level||<0.06 EU/ml as measured by gel clot LAL assay|
|Formulation||PODS™ were lyophilized from a volatile solution|
PODS™ protein crystals may be reconstituted at 200 million PODS™/ml in water. 20% glucose has a buoyant density closer to PODS™ protein crystals and can be useful for aliquoting.
PODS™ protein crystals are highly stable when stored in aqueous solution (pH range 6 - 8).
|Stability and Storage||Upon receipt, store at 4°C. PODS™ protein crystals are stable for at least 1 year when dry and 6 months when resuspended.|