Recombinant Human BDNF

Code Description Price Qty
GFH1-2 Recombinant Human BDNF, 2 μg £44.21
GFH1-10 Recombinant Human BDNF, 10 μg £105.00
GFH1-100 Recombinant Human BDNF, 100 μg £395.00
GFH1-500 Recombinant Human BDNF, 500 μg £1,950.00
GFH1-1000 Recombinant Human BDNF, 1 mg £3,500.00
Induced proliferation of C6 cells for Human BDNF. Cell proliferation was measured to calculate the ED50, which is as expected less than 2 μg/ml.
Non-reducing (-) and reducing (+) conditions in a 4 - 20% Tris-Glycine gel stained with Coomassie Blue. 1 μg of protein was loaded in each lane. Human BDNF is a non-covalent homodimer and has a predicted Mw of 13.6 kDa under both reducing and non-reducing conditions.

This growth factor is also available as PODS™ growth factors for sustained release, see here.

Description

Brain-derived neurotrophic factor (BDNF) is a nerve growth factor that binds two receptors, the low-affinity nerve growth factor receptor (LNGFR) and the tropomyosin receptor kinase B (TrkB), to support neuron growth and survival. BDNF expression in the hippocampus is essential for long-term memory storage and learning. Some protein domains of BDNF are identical with those of NGF and another neurotrophic factor, designated NT-3 (Neurotrophin-3). Human, mouse, rat, and pig BDNF are cross-reactive.

Activity Assay

Human BDNF is fully biologically active when compared to standard. The activity is determined by the proliferation of C6 cells and it is typically less than 2 μg/ml. This corresponds to an expected specific activity of 5.0 x 102 units/mg.

AA Sequence

MHSDPARRGE LSVCDSISEW VTAADKKTAV DMSGGTVTVL EKVPVSKGQL KQYFYETKCN PMGYTKEGCR GIDKRHWNSQ CRTTQSYVRA LTMDSKKRIG WRFIRIDTSC VCTLTIKRGR

Alternative Names

Brain-derived neurotrophic factor, neurotrophin, abrineurin
Product Details
Length 120 aa
Molecular Weight 27.2 kDa
Structure Monomer
Source E. coli
Accession Number P23560
Purity ≥95% determined by reducing and non-reducing SDS-PAGE
Endotoxin Level ≤1.00 EU/μg as measured by kinetic LAL
Formulation Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA)
Reconstitution Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Stability and Storage

12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed.

Citations

The kinase function of MSK1 regulates BDNF signaling to CREB and basal synaptic transmission, but is not required for hippocampal long-term potentiation or spatial memory. Daumas, S, et al. 2017. eNeuro.

Characterization of energy and neurotransmitter metabolism in cortical glutamatergic neurons derived from human induced pluripotent stem cells: A novel approach to study metabolism in human neurons. Aldana, BI, et al. 2017. Neurochemistry International.