Recombinant Human IL-6
Induced proliferation of 7TD1 cells for Human IL-6. Cell proliferation was measured to calculate the ED50, which is as expected less than 250 pg/ml.
Non-reducing (-) and reducing (+) conditions in a 4 - 20% Tris-Glycine gel stained with Coomassie Blue. 1 μg of protein was loaded in each lane. Human IL-6 has a predicted Mw of 21 kDa.
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PODS® Growth Factors
This growth factor is also available as PODS® for sustained release, see here.Description
Interleukin-6 (IL-6) is a cytokine that is expressed by T cells, macrophages, and muscle cells. IL-6 acts to stimulate an immune response upon infection or trauma. IL-6 has both pro-inflammatory and anti-inflammatory functions and signals through the cell-surface type 1 cytokine receptor complex containing the IL-6Ra and gp130 chains. Human IL-6 is active on mouse and rat cells.Activity Assay
Human IL-6 is fully biologically active when compared to standard. The activity is determined by the ability to induce 7TD1 cells proliferation and it is typically less than 250 pg/ml. This corresponds to an expected specific activity of 4 x 106 units/mg. The activity can also be determined by the ability to induce B9 cells proliferation and it is typically less than 25 pg/ml, for an expected specific activity of 4 x 107 units/mg.AA Sequence
MPVPPGEDSK DVAAPHRQPL TSSERIDKQI RYILDGISAL RKETCNKSNM CESSKEALAE NNLNLPKMAE KDGCFQSGFN EETCLVKIIT GLLEFEVYLE YLQNRFESSE EQARAVQMST KVLIQFLQKK AKNLDAITTP DPTTNASLLT KLQAQNQWLQ DMTTHLILRS FKEFLQSSLR ALRQMAlternative Names
Interleukin-6, interleukin 6, IL6, IL 6, IFN-β2, interferon β 2, B-cell differentiation factor, BSF-2, HSF, MGI-2, CTL differentiation factor| Product Details | |
|---|---|
| Length | 185 aa |
| Molecular Weight | 21 kDa |
| Structure | Monomer |
| Source | E. coli |
| Accession Number | P05231 |
| Purity | ≥95% determined by reducing and non-reducing SDS-PAGE |
| Endotoxin Level | ≤1.00 EU/μg as measured by kinetic LAL |
| Formulation | Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, pH 7.5 |
| Reconstitution | Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions. |
| Stability and Storage |
12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed. |
