Recombinant Human SHH

Code Description Price Qty
GFH168-5 Recombinant Human SHH, 5 μg £44.21
GFH168-25 Recombinant Human SHH, 25 μg £105.00
GFH168-100 Recombinant Human SHH, 100 μg £262.50
GFH168-1000 Recombinant Human SHH, 1 mg £1,700.00

This growth factor is also available as PODS™ growth factors for sustained release, see here.

Description

Sonic hedgehog (SHH) is a member of a small group of hedgehog secreted proteins that are essential for development in both vertebrates and invertebrates. There are three mammalian hedgehog homologues, sonic, desert, and indian, that signal via the Patched-1 and Patched-2 receptors. SHH is a morphogen that is essential during vertebrate organogenesis and adult stem cell division.

Activity Assay

Human SHH is fully biologically active when compared to standard. The activity is determined by the dose-dependent induction of alkaline prosphatase production by C3H/10T1/2 fibroblasts and it is typically 750 ng/ml.

AA Sequence

MIIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKISRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRALDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSV AAKSGGCFP

Alternative Names

Sonic hedgehog, HHG-1, HHG1, HLP3, HPE3
Product Details
Length 179 aa
Molecular Weight 20.2 kDa
Structure Monomer
Source E. coli
Accession Number Q15465
Purity ≥95% determined by reducing and non-reducing SDS-PAGE
Endotoxin Level ≤1.00 EU/μg as measured by kinetic LAL
Formulation Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, pH 7.5
Reconstitution Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Stability and Storage

12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed.