Recombinant Human TGF-β1

Code Description Price Qty
GFH39-5 Recombinant Human TGF-β1, 5 μg £50.00
GFH39-20 Recombinant Human TGF-β1, 20 μg £185.00
GFH39-100 Recombinant Human TGF-β1, 100 μg £685.00
GFH39-500 Recombinant Human TGF-β1, 500 μg £1,995.00
GFH39-1000 Recombinant Human TGF-β1, 1 mg £3,400.00
Non-reducing (-) and reducing (+) conditions in a 4 - 20% Tris-Glycine gel stained with Coomassie Blue. 1 μg of protein was loaded in each lane. Human TGF-β1 has a predicted Mw of 25.6 kDa (each monomer is 12.8 kDa).

This growth factor is also available as PODS™ growth factors for sustained release, see here.

Description

Transforming Growth Factor β 1 (TGF-β1) is a member of the TGF-β superfamily of cytokines. Members of this family exhibit regulatory activity in immunity, proliferation, adhesion, migration, and growth inhibition pathways. TGF-β1 signals through SMAD proteins via the TGF-β RI and TGF-β RII receptors.

Activity Assay

Inhibition of IL-4-induced HT-2 cell proliferation

AA Sequence

ALDTNYCFSS TEKNCCVRQL YIDFRKDLGW KWIHEPKGYH ANFCLGPCPY IWSLDTQYSK VLALYNQHNP GASAAPCCVP QALEPLPIVY YVGRKPKVEQ LSNMIVRSCK CS

Alternative Names

Transforming Growth Factor β 1, TGF β 1, TGF-β-1, TGFB1, TGFβ1, differentiation inhibiting factor, cartilage-inducing factor
Product Details
Length 112 / 224 aa
Molecular Weight 12.8 / 25.6 kDa
Structure Dimer
Source CHO cells
Accession Number P01137
Purity ≥95% determined by reducing and non-reducing SDS-PAGE
Endotoxin Level ≤1.00 EU/μg as measured by kinetic LAL
Formulation Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% trifluoroacetic acid (TFA) and trehalose (20:1 trehalose to protein ratio)
Reconstitution Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile 10 mM HCl at 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Stability and Storage

12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed.