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PeptiGels®

 

Click the image to see the growing range of successfully cultured cells 

Extensively validated in over 40 cell types, PeptiGel® is a family of defined, tunable, synthetic self-assembling peptide hydrogels (SAPHs) that provide a perfect canvas for 3D cell culture and bioprinting. Bespoke. Not one-size-fits-all, PeptiGel® hydrogel's fully defined components enable consistent product quality and reproducible results. This provides a tailored, better, kinder alternative to traditional animal-derived hydrogels. 

Next-generation self-assembling peptide hydrogel (SAPHs) 

Peptide stacks that form in PeptiGel develop 3D nano-fibrous matrix hydrogel networks 

Tunable in two key ways

PeptiGel Self-Assembling Peptides Technology

Click the image to see the growing range of successfully cultured cells 

PeptiGel is a family of related hydrogels that provide a range of class-leading SAPHs and bioinks to support your cell culture applications. The proprietary hydrogel technology allows us to tune mechanical and functional properties, to provide you with the most suitable materials tailored to your application's needs. Firstly, the peptide backbone sequence is modified affecting properties such as charge. Secondly, motifs, such as RGD and GFOGER are fused to the backbone sequence completely interesting them into the hydrogel. This is not an admixture. Of course, PeptiGels may be diluted to optimize viscosity. You can be assured that PeptiGels® are physiological and biologically relevant hydrogels that mimic the cell micro-environment and provide a synthetic extracellular matrix. 

Trial sizes for new users 

Up to 5 different 1 ml packs may be purchased at an introductory price (see below) for evaluation with your culture system.

PeptiInk® for 3D bioprinting

Specifically designed to facilitate the 3D printing of cells. Available for Alpha 4 and Alpha 4 PLUS only.

Just a few of the cell types cultured with PeptiGel®

Cell cultured with PeptiGel

Examples of cells grown with PeptiGel® hydrogels. PDAC= pancreatic ductal adenocarcinoma, rOSFs = rat oesophageal stromal fibroblasts. For further details, please see the PeptiGel resources page.

PeptiGel® - What researchers say

Prof Manuel Salmeron-Sanchez QuoteDr Adam Reid QuoteDr Angela Imere QuoteProf Julie Gough QuoteDr Marco Domingos QuoteDr Armando Del Rio Hernandez Quote

Cell Compatibility

Gel type and demonstrated compatible cells 

PeptiGel® Type

Charge

Stiffness (kPa)

Biomimetic Functionality

Demonstrated Compatible Primary Cells and Cell Lines 

Alpha 1

Neutral 3 - 5 None

Adipose-derived stem cellsChondrocytesDorsal root ganglion neuronsBreast MCF7, MCF10a, MDAMB231 and Breast EpH4, Schwann cells, Oesophageal, Fibroblast 3T3, Kidney HEK293, iPSCs, Ovary A2780 and SK-OV-3

Alpha 2 +1 6 - 8 None Adipose-derived stem cellsiPSC-derived cardiomyocytesChondrocytesDorsal root ganglion neuronsLiver HepG2Pancreas Suit-2, Prostate PC3, Prostate pNT2, Ovary A2780 and SK-OV-3Hepatic cells

Alpha 2 Plus

+1 6 - 8 RGD & GFOGER in vivo Neuronal
Alpha 4 +2 0.7 - 1.3 None Mammary epithelial, Bone marrow-derived stem cells, Dermal fibroblasts, Colon organoidsKidney organoidsSynovial cellsiPSCs, Fibroblasts 3T3 + L929, Muscle C2C12, Ovary A2780 and SK-OV-3, HUVECS 
Alpha 4 Plus +2 0.7 - 1.3 RGD & GFOGER Stromal fibroblasts, Ovary A2780 and SK-OV-3Colon crypts
Alpha 8 -3 ~ 0.25 None Hepatic Cells
Delta 1 Neutral 3 - 5 None Mesenchymal Stem Cells
Gamma 2  +1 ~ 1.5  None Dorsal root ganglion neuronsPancreas Suit-2Hepatic cellsiPSC
Gamma 2 Plus +1  ~ 2.5  RGD & GFOGER Neuronal
Gamma 4 +2 0.175  - 0.35  None For cells requiring a softer environment, including primary cells, immortalized cells and stem cells
Gamma 4 Plus +2 0.175 - 0.35 RGD & GFOGER Spheroids and organoids

View table sorted by PeptiGel-validated cell line (opens new window)

The stiffness values given here are indicative and have been measured by shear rheometry after 24 incubation in DMEM media.

Bespoke Peptide Hydrogel Design

The chemistry, mechanical and bio-functional properties of each PeptiGel® can be tuned to create bespoke products to suit your cells’ needs. This includes functionalizing a PeptiGel® with peptide sequences from collagen (GFOGER), fibronectin (RGD), & laminin (IKVAV or YIGSR). We can also tailor the material properties to precisely fit your requirements for a wide range of application areas, including cell therapies, drug delivery, and high-throughput screening.

If you require specific material design characteristics or assistance choosing the PeptiGel® that's most suitable for your application, please get in touch.

PeptiGel Technology GuidePeptiGel Resources

Products

PeptiGel® Alpha 1™ $40.00
PeptiGel® Alpha 2™ $40.00
PeptiGel® Alpha 2 PLUS™ $48.00
PeptiGel® Alpha 4™ $40.00
PeptiGel® Alpha 4 PLUS™ $48.00
PeptiInk® Alpha 4™ $40.00
PeptiInk® Alpha 4 PLUS™ $48.00
PeptiGel® Alpha 8™ $40.00
PeptiGel® Gamma 2™ $40.00
PeptiGel® Gamma 4™ $40.00
PeptiGel® Delta 1™ $40.00
PeptiGel® Gamma 2 PLUS™ $48.00
PeptiGel® Gamma 4 PLUS™ $48.00