Exo-spin™ blood
Product description
Exo-spin™ technology combines precipitation and size exclusion chromatography (SEC), making it superior to techniques that rely solely on one method. Using only precipitation for exosome isolation will result in co-purification of large amounts of non-exosomal proteins and other material as well as carryover of the precipitant. SEC is reliable for exosome isolation, but a precipitation step is needed to concentrate your sample prior to SEC isolation. If there is no precipitation, a lower exosome recovery will be observed. We provide a simple two-step protocol which allows you to purify your sample in less than 2 hours with consistent and reliable results!
The EX02 Exo-spin™ kit has been developed to process small volumes of blood samples (both plasma and sera). The kit includes Exo-spin™ buffer as well as SEC columns. The Exo-spin™ buffer is a polymer-based precipitation buffer and will be used for the first step. For the second step, pre-packed and equilibrated ready-to-use SEC columns are included.
Which Exo-spin™ kit shall I choose for my sample volume?
† Highly concentrated exosome samples (e.g. 1x1012 particles/ml) other than blood can also be used.
Exo-spin™ column and resin pore size
The column bed volume is 500 µl, allowing for 100 µl of volume to be loaded on top of the column.
The pore size of the resin is approximatively 30 nm to attain a highly pure exosome elution. All other molecules (e.g. proteins, lipids) which are smaller than 30 nm will enter into the pores and remain trapped in the column.
Highest recovery and purity
As mentioned above, essentially all proteins and lipids will be retained in the column and will elute later than the exosomes, ensuring a highly pure sample ready for your downstream application.
Reproducibility
All our columns are manufactured in our laboratory to ensure a high reproducibility between each lot. As proof of reproducibility and batch-to-batch consistency, a large number of peer-reviewed scientific papers have been published describing the use of Exo-spin™.
Storage
Upon receipt, store purification columns and Exo-spin™ Buffer at 4°C. All other components should be stored at room temperature (15°C - 25°C).
Frequently Asked Questions (FAQs)
For any additional questions, please refer to FAQs document below.
Start today! Select our starter pack
We designed the ideal starter pack to guide your exosome research. The starter pack includes the exosome purification kit of your choice, exosome validated antibodies, and NTA profiling analysis. The complete details can be found in the product page here.
Product data
EX02 Exo-spin™ kit comparative data
Data determined by nanoparticle tracking analysis (NTA). Each curve represents the average of 3 technical replicate measurements for each exosome isolation method and biofluid triplicate experiment. (PM = Precipitation Method). Figure taken and adapted from (Martins, TS et al., 2018).
Data determined by NTA and EXOCET. Serum sample has been analysed for qualitative comparison between Exo-spin™ and precipitation methods.
Characterizing Exo-spin™ isolated exosomes using nanoparticle tracking analysis (NTA)
Exosome characterization with NTA. Exosomes have been isolated using the Exo-spin™ kit and analysis performed with the ZetaView® instrument.
Downstream applications: advice and content
The EX02 Exo-spin™ kit is compatible with all downstream application and has been published in a large range of different applications.
RNA analysis:
- Wang L., Wang C., Jia X. and Yu J. (2018). Circulating Exosomal miR-17 Inhibits the Induction of Regulatory T Cells via Suppressing TGFBR II Expression in Rheumatoid Arthritis. Cellular Physiology and Biochemistry; 50:1754–1763.
- Emanueli C., Shearn AI., Laftah A., Fiorentino F., Reeves BC., Beltrami C., Mumford A., Clayton A., Gurney M., Shantikumar S. and Angelini G. (2016). Coronary Artery-Bypass-Graft Surgery Increases the Plasma Concentration of Exosomes Carrying a Cargo of Cardiac MicroRNAs: An Example of Exosome Trafficking Out of the Human Heart with Potential for Cardiac Biomarker Discovery. PLoS One 29;11(4):e0154274.
Mass Spectrometry:
- Menezes-Neto A., Fidalgo Sáez MJ., Lozano-Ramos I., Segui-Barber J., Martin-Jaular L., Estanyol Ullate JM., Fernandez-Becerra C., Borrás FE., and del Portillo HA. (2015). Size-exclusion chromatography as a stand-alone methodology identifies novel markers in mass spectrometry analyses of plasma-derived vesicles from healthy individuals. Journal of Extracellular Vesicles; 4: 10.3402/jev.v4.27378.
Functional study:
- Sheller-Miller S, Trivedi J, Yellon SM, and Menon R. (2019). Exosomes Cause Preterm Birth in Mice: Evidence for Paracrine Signaling in Pregnancy. Scientific Reports 24;9(1):608.
Citations
Sheller-Miller S, Trivedi J, Yellon SM, and Menon R. (2019). Exosomes Cause Preterm Birth in Mice: Evidence for Paracrine Signaling in Pregnancy. Scientific Reports 24;9(1):608.
Soares Martins, T., Catita, J., Martins Rosa, I., Cruz da Silva, O., and Henriques, A.G. (2018) Exosome isolation from distinct biofluids using precipitation and column-based approaches. PLoS One. 13(6):e0198820.
Wang L., Wang C., Jia X. and Yu J. (2018). Circulating Exosomal miR-17 Inhibits the Induction of Regulatory T Cells via Suppressing TGFBR II Expression in Rheumatoid Arthritis. Cellular Physiology and Biochemistry; 50:1754–1763.
Emanueli C., Shearn AI., Laftah A., Fiorentino F., Reeves BC., Beltrami C., Mumford A., Clayton A., Gurney M., Shantikumar S. and Angelini G. (2016). Coronary Artery-Bypass-Graft Surgery Increases the Plasma Concentration of Exosomes Carrying a Cargo of Cardiac MicroRNAs: An Example of Exosome Trafficking Out of the Human Heart with Potential for Cardiac Biomarker Discovery. PLoS One 29;11(4):e0154274
Menezes-Neto A., Fidalgo Sáez MJ., Lozano-Ramos I., Segui-Barber J., Martin-Jaular L., Estanyol Ullate JM., Fernandez-Becerra C., Borrás FE., and del Portillo HA. (2015). Size-exclusion chromatography as a stand-alone methodology identifies novel markers in mass spectrometry analyses of plasma-derived vesicles from healthy individuals. Journal of Extracellular Vesicles; 4: 10.3402/jev.v4.27378.
