Exo-spin™ blood
Product Description
Exo-spin™ technology generates superior yields and higher quality whole exosomes using a unique combination of precipitation and size exclusion chromatography. No ultracentrifugation is required. No organic buffers. The Exo-spin™ (EX01) kit is suitable for the preparation of pure, functional exosomes from a variety of biological fluids including cell culture media, urine, and saliva. For blood plasma and sera, Exo-spin™ blood (EX02) is an ideal choice.
Benefits
Exosomes purified using Exo-spin™ are suitable for a variety of downstream applications including RNA analysis, protein analysis and functional use.
Simple, easy-to-follow two-hour protocol.
(1) Remove cells and cellular debris.
(2) Pellet microvesicle containing fraction.
(3) Purify exosomes.
Choose the right kit for your samples
The best kit to use depends on both your sample type and the downstream application. You can download simple guide to choosing your Exo-spin™ kit here.
Product Data
NTA measurement of samples prepared from cell culture medium (a) Exo-spin™ yields significantly higher numbers of vesicles of the expected 40-120 nm size range than alternatives including (b) ultracentrifugation (c) competitor kit. The size distribution profile obtained with Exo-spin™ most closely resembles ultra-centrifugation.
Lower rRNA contamination levels Analysis on Bioanalyzer instrument shows RNA preps (Trizol) following (d) Exo-spin™ purification from cell culture samples compared with (e) ultracentrifugation or (f) competitor kit.
References
Santangelo L, Giurato G, Cicchini C, Montaldo C, Mancone C, Tarallo R, Battistelli C, Alonzi T, Weisz A, and Tripodi M. The RNA-Binding Protein SYNCRIP Is a Component of the Hepatocyte Exosomal Machinery Controlling MicroRNA Sorting. (2016). Cell Reports 17(3): 799-808.
Welton JL, Webber JP, Botos LA, Jones M, Clayton A. Ready-made chromatography columns for extracellular vesicle isolation from plasma. (2015). J Extracellular Vesicles 4: 27269.
Lane RE, Korbie D, Anderson W, Vaidyanathan R, Trau M. Analysis of exosome purification methods using a model liposome system and tunable-resistive pulse sensing. (2015). Scientific Reports 5: 7639.
