PODS® Human Wnt-1
PODS® TechnologyPODS® proteins are made using an insect cell expression system in which the active protein is co-expressed alongside polyhedrin carrier protein. Polyhedrin forms microcrystals within insect cells which specifically capture the active protein to form a co-crystal complex. The active protein is captured in its nascent, natively folded form with limited scope for proteolytic degradation. Consequently, excellent levels of bioactivity are observed. The PODS® co-crystals provide a sustained release mechanism and can be used to functionalize surfaces. For further details, please refer to the PODS® Technology page.
Product DescriptionThe product contains the polyhedrin protein co-crystalized with Human Wnt-1. Wnt proteins constitute a large family of secreted proteins (sharing 20% to 85% aminoacid identity) with different roles in cell fate deciosion, axon guidance, and tumour formation. Wnt-1 is a secreted glycoprotein that binds members of the Frizzled family of cell surface receptors and plays a crucial role in the embryonic development and carcinogenesis. Wnt-1 signaling is responsible for stabilizing the intracellular signaling protein β-catenin. For certain cell lines, Wnt-1 supports self-renewal while keeping undifferentiated.
PODS® co-crystals provide a depot of proteins which are steadily secreted. It has been estimated that the biological activity of 50 million PODS® co-crystals generates the same peak dose as 3.3 μg of standard recombinant protein. However, at 5 days following the start of seeding the PODS® co-crystals, there are more than 50% of these peak levels still present in the culture system. Ultimately, the amount of PODS® co-crystals that is optimal for a particular experiment should be determined empirically. Based on previous data, we suggest using 50 million PODS® co-crystals in place of 3.3 μg of standard growth factor as a starting point."
To control for cross-reactivity with cells or as a negative control, we recommend using PODS® growth factors alongside PODS® Empty crystals, as the latter do not contain or release cargo protein.
AA SequenceMADVAGTSNR DFRGREQRLF NSEQYNYNNS KNSRPSTSLY KKAGFANSSG RWWGIVNVAS STNLLTDSKS LQLVLEPSLQ LLSRKQRRLI RQNPGILHSV SGGLQSAVRE CKWQFRNRRW NCPTAPGPHL FGKIVNRGCR ETAFIFAITS AGVTHSVARS CSEGSIESCT CDYRRRGPGG PDWHWGGCSD NIDFGRLFGR EFVDSGEKGR DLRFLMNLHN NEAGRTTVFS EMRQECKCHG MSGSCTVRTC WMRLPTLRAV GDVLRDRFDG ASRVLYGNRG SNRASRAELL RLEPEDPAHK PPSPHDLVYF EKSPNFCTYS GRLGTAGTAG RACNSSSPAL DGCELLCCGR GHRTRTQRVT ERCNCTFHWC CHVSCRNCTH TRVLHECL
Alternative NamesWingless type 1, Wnt 1, Wnt1, INT-1, Wnt-1 proto-oncogene protein (precursor)
Research Use OnlyThis product is for Research Use Only.
|Molecular Weight||43.5 kDa|
|Source||Spodoptera frugiperda (Sf9) cell culture|
|Endotoxin Level||<0.06 EU/ml as measured by gel clot LAL assay|
|Formulation||PODS® were lyophilized from a volatile solution|
PODS® co-crystals may be reconstituted at 200 million co-crystals/ml in water. 20% glucose has a buoyant density closer to PODS® co-crystals and can be useful for aliquoting.
PODS® co-crystals are highly stable when stored in aqueous solution (pH range 6 - 8).
|Stability and Storage||Upon receipt, store at 4°C. PODS® co-crystals are stable for at least 1 year when dry and 6 months when resuspended.|