Code Description Price Qty
PPH1-50 PODS™ Human BDNF, 50 million £95.00
PPH1-250 PODS™ Human BDNF, 250 million £295.00
PPH1-1000 PODS™ Human BDNF, 1 billion £995.00
PODS™ co-crystals
PODS™ co-crystals

PODS™ Technology

PODS™ proteins are made using an insect cell expression system in which the active protein is co-expressed alongside polyhedrin carrier protein. Polyhedrin forms microcrystals within insect cells which specifically capture the active protein to form a co-crystal complex. The active protein is captured in its nascent, natively folded form with limited scope for proteolytic degradation. Consequently, excellent levels of bioactivity are observed. The PODS™ co-crystals provide a sustained release mechanism and can be used to functionalize surfaces. For further details, please refer to the PODS™ Technology page.

Product Description

The product contains the polyhedrin protein co-crystalized with Human BDNF. Brain-derived neurotrophic factor (BDNF) is a nerve growth factor that binds two receptors, the low-affinity nerve growth factor receptor (LNGFR) and the tropomyosin receptor kinase B (TrkB), to support neuron growth and survival. BDNF expression in the hippocampus is essential for long-term memory storage and learning. Some protein domains of BDNF are identical with those of NGF and another neurotrophic factor, designated NT-3 (Neurotrophin-3). Human, mouse, rat, and pig BDNF are cross-reactive.

Usage Recommendation

PODS™ co-crystals provide a depot of proteins which are steadily secreted. It has been estimated that the biological activity of 50 million PODS™ co-crystals generates the same peak dose as 3.3 μg of standard recombinant protein. However, at 5 days following the start of seeding the PODS™ co-crystals, there are more than 50% of these peak levels still present in the culture system. Ultimately, the amount of PODS™ co-crystals that is optimal for a particular experiment should be determined empirically. Based on previous data, we suggest using 50 million PODS™ co-crystals in place of 3.3 μg of standard growth factor as a starting point."

To control for cross-reactivity with cells or as a negative control, we recommend using PODS™ growth factors alongside PODS™ Empty crystals, as the latter do not contain or release cargo protein.

AA Sequence


Alternative Names

Brain-derived neurotrophic factor, neurotrophin, abrineurin
Product Details
Length 164 aa
Molecular Weight 38 kDa
Structure Dimer
Source Spodoptera frugiperda (Sf9) cell culture
Accession Number P23560
Endotoxin Level <0.06 EU/ml as measured by gel clot LAL assay
Formulation PODS™ were lyophilized from a volatile solution

PODS™ co-crystals may be reconstituted at 200 million co-crystals/ml in water. 20% glucose has a buoyant density closer to PODS™ co-crystals and can be useful for aliquoting.

PODS™ co-crystals are highly stable when stored in aqueous solution (pH range 6 - 8).

Stability and Storage Upon receipt, store at 4°C. PODS™ co-crystals are stable for at least 1 year when dry and 6 months when resuspended.


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Rey FA. Virology: Holed up in a natural crystal. (2007) Nature. 446: 35-37.

Mori H. Immobilization of Bioactive Growth Factors into Cubic Proteinous Microcrystals (Cypovirus Polyhedra) and Control of Cell Proliferation and Differentiation. (2010) NSTI-Nanotech. 3: 222-225.

Satoshi Abe, Hiroshi Ijiri, Hashiru Negishi, Hiroyuki Yamanaka, Katsuhito Sasaki, Kunio Hirata, Hajime Mori, and Takafumi Ueno. Design of Enzyme-Encapsulated Protein Containers by In-Vivo Crystal Engineering. (2015) Advanced Materials. 27(48): 7951-7956.