Recombinant Human SDF-1 β / CXCL12b

Code Description Price Qty
GFH159-2 Human SDF-1 β, 2 μg $65.00
GFH159-10 Human SDF-1 β, 10 μg $165.00
GFH159-100 Human SDF-1 β, 100 μg $410.00
GFH159-1000 Human SDF-1 β, 1 mg $2,950.00
Induced chemotaxis of human primary T cells assay for Human SDF-1 β. Cells that migrated were counted using a luminescent substrate. Migration over basal levels was reported in response to Human SDF-1 β starting at 5 ng/ml.
Non-reducing (-) and reducing (+) conditions in a 4 - 20% Tris-Glycine gel stained with Coomassie Blue. 1 μg of protein was loaded in each lane. Human SDF-1 β has a predicted Mw of 8.5 kDa.

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Description

Stromal cell-derived factor-1 β (SDF-1 β), also known as CXCL12b, is one of two SDF-1 splice variants made by a wide variety of cells upon stimulation by inflammatory cytokines such as TNF, IL-1, and LPS. SDF-1 β signals through the G protein-coupled receptor CXCR4 to recruit activated leukocytes.

Activity Assay

Human SDF-1 β is fully biologically active when compared to standard. The activity is determined by the ability to induce chemotaxis of human primary T cells.

AA Sequence

KPVSLSYRCP CRFFESHVAR ANVKHLKILN TPNCALQIVA RLKNNNRQVC IDPKLKWIQE YLEKALNK

Alternative Names

Monocyte Chemotactic Protein 1, CCL2, JE, MCAF
Product Details
Length 68 aa
Molecular Weight 8.0 kDa
Structure Monomer
Source E. coli
Accession Number P48061 (2)
Purity ≥95% determined by reducing and non-reducing SDS-PAGE
Endotoxin Level ≤1.00 EU/μg as measured by kinetic LAL
Formulation Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA)
Reconstitution Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Stability and Storage

12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed.