Code Description Price Qty
M09-50 SOS® neuronal supplement, 50 ml $294.00


SOS® neuronal supplement is part of the LiveLight™ cell culture system encompasses three different photostable products:

  • MEMO® medium replaces DMEM
  • NEUMO® medium replaces Neurobasal® media
  • SOS® supplement replaces B-27® and similar neuronal supplements (e.g. N2, SATO, NS21).  

LiveLight™ cell culture products do more than just reduce auto-fluorescence. They actively protect your cells from the lethal effects of photo-induced free radicals that are common components of standard media. Experiments with cultured cells, including fluorescence microscopy, optogenetics, fluorescence activated cell sorting (FACS), calcium imaging, super-resolution microscopy and automated cell culture, often entail high levels or prolonged exposure to light. However, cell culture media and supplements contain components that are converted to toxic free radicals by light. In particular, DMEM (Dulbecco’s Modified Eagle Medium) and Neurobasal® medium, as well as cell culture supplements such as B-27®, SATO and NS21, can lead to significant perturbation ofbehavior behaviour and marked increases in cell death. This issue has been discussed, for example, in “Artifacts of Light”, Nature Methods (2013) volume 10 (12) page 1135. 


LiveLight cell culture media and supplements have been reformulated with specific phototoxic components eliminated and replaced. 


LiveLight™ media and supplements allow prolonged exposure of cells to light whilst maintaining high levels of cell viability and functionality. As shown below, this allows the generation of remarkably improved experimental data. 

Product Data


7-day old primary rat neuronal cells cultured in standard media or LiveLight™ media in dark and light conditions. Cells were stained with neuronal markers Tuj1 and GFAP, and with DAPI, a marker of cell viability. Light conditions result in widespread cell death with standard media whereas cells cultured in LiveLight™ media show no decrease in viability.



Successful FACS sorting, culturing and differentiation of viable GFP and RFP-labeled central nervous system stem cells (OPCs) from adult mouse brain. (A) Neurobasal® and B-27® destroy cell viability (B) Cells maintained in NEUMO® and SOS® retain viability. Data courtesy of Abbe Crawford and Prof. Robin Franklin of the Wellcome Trust-MRC Cambridge Stem Cell Institute. Scale bar = 100 mm.



OPCs (A,B), Cortical Neurons (C) and Hippocampal Neurons (D) grownin standard conditions and in LiveLight™ media.


Dramatic improvements in image quality can routinely be achieved when using LiveLight™.

The following were kindly provided by Dr. Sami Barmada's lab, University of Michigan. All images were obtained with cells maintained in NEUMO® and SOS®.


Primary rodent cortical neurons expressing mApple (green) and a mutant gene (Matrin 3) involved in familial amyotrophic lateral sclerosis, labeled with EGFP (red), stained with a nuclear dye (Hoechst, purple). 40X magnification. Image provided by Ye-Shih Ho.


Live primary rodent cortical neurons expressing mApple (red) and a mutated gene (C9orf72, green) involved in familial amyotrophic lateral sclerosis and frontotemporal dementia, stained with a nuclear dye (Hoechst, cyan). 40X magnification. Image provided by Brittany Flores.



Neuronal cells imaged in the presence of Neurobasal medium. The same neuronal cells in the presence of NEUMO® and SOS® under the same illumination and photographic conditions. Courtesy Prof Sami Bamarda, University of Michigan.


  • John H. Stockley, Kimberley Evans, Moritz Matthey, Katrin Volbracht, Sylvia Agathou, Jana Mukanowa, Juan Burrone & Ragnhildur T. Káradóttir. Surpassing light-induced cell damage in-vitro with novel cell culture media (2017) Scientific Reports
  • Flores BN, Li X, Malik AM, Martinez J, Beg AA, Barmada SJ. (2019). An Intramolecular Salt Bridge Linking TDP43 RNA Binding, Protein Stability, and TDP43-Dependent Neurodegeneration. Cell Reports 23;27(4):1133-1150.e8.
  • Duke CG, Savell KE, Phillips RA, Day JJ. (2019). Blue light-induced gene expression alterations in cultured neurons are the result of phototoxic interactions with neuronal culture media. bioRxiv.
  • Green KM, Sheth U, Flores BN, Wright SE, Sutter A, Kearse MG, Barmada S, Ivanova MI, Todd PK. (2019). High-throughput screening yields several small-molecule inhibitors of repeat-associated non-AUG translation. Journal of Biological Chemistry 23.
  • Weskamp K, Tank EM, Miguez R, Gómez NB, White M, Lin Z, Gonzalez CM, Serio A, Sreedharan J, Barmada SJ (2019). Neuronal hyperexcitability drives TDP43 pathology by upregulating shortened TDP43 protein isoforms. bioRxiv.