Simplified Tangential Flow Filtration
The EVlution™ TFF System delivers high-yield, high-purity purification of a range of biomolecules from up to 3 litres of starting material - in just 90 minutes. EVlution™ is modular, affordable, easy to use, and requires minimal hands-on time, freeing you to focus on what matters most: your research.
Its innovative SwitchFlow™ technology reduces retentate volume to under 10 mL, maximising recovery while minimising loss. The compact, modular design supports 10 interchangeable polyethersulfone hollow fibre cartridge pore sizes. Filters are reusable up to 20 times, keeping running costs low. For extracellular vesicle work, EVlution™ pairs seamlessly with Exo-spin™ purification columns for a complete, end-to-end workflow.
Whatever your biomolecule, EVlution™ has you covered.
Exploring how TFF is used to purify proteins: the range of proteins it handles, how much diafiltration a given job needs, whether to run that diafiltration continuously or discontinuously, and how the membrane itself interacts with the protein.
How tangential flow filtration grew from 1960s ultrafiltration membranes into a core step in dairy, biopharma, mRNA-LNP and EV processing,
CellGS launches EVlution Pro, an automated benchtop TFF instrument for concentrating and buffer exchanging EVs, proteins, antibodies, nucleic acids and viruses.
How does freeze-thawing affect EVs? How much is each of the common characterisation methods affected? How does sample type influence the results, and what can be done to reduce these effects?
How sample type, volume and membrane choice set the real capacity of a tangential flow filtration run, from conditioned media and biofluids to viral vectors, nucleic acids, proteins and synthetic nanoparticles, at laboratory and pilot scale
Most tangential flow filtration (TFF) protocols for extracellular vesicles (EVs) use the membrane as a net. Everything bigger than the cut-off stays in the retentate, everything smaller goes to waste, and the EVs come out as a single concentrated population. TFF can also be used to gate EVs based on their size.
As we launch our first plant-derived growth factor, hTGF-β3, we take a look at the (tobacco) field.
EV storage is a topic we are often asked about: Which buffers, what temperatures, and for how long? What is the impact on downstream applications of the EVs?
