EVlution TFF
EVlution™ is an affordable benchtop dedicated instrument that makes tangential flow filtration (TFF) accessible to researchers. EVlution™ utilizes polyethersulfone hollow fibres to create a highly selective barrier that enables concentration and isolation of extracellular vesicles (EVs). TFF is a more efficient method for concentrating large volumes compared to traditional dead-end filtration. EVlution allows researchers to concentrate and purify EVs from large volumes of starting material.
Simple Principles and Workflow
EVlution™ is a compact TFF platform designed for efficient size-dependent concentration and purification of EVs and other molecules. TFF provides an effective method of size-based separation of EVs from other biomolecules, whilst minimizing membrane fouling. The characteristics of TFF design makes it an excellent choice for workflows at the lab scale. The adaptable design of EVlution™ supports a wide range of hollow fiber filter configurations, making it a flexible solution for diverse laboratory-scale applications. With an intuitive interface and streamlined operation, EVlution™ simplifies complex filtration processes while delivering reliable performance.
The blue path represents the flow of the sample entering through the inlet, across the TFF cartridge and looping back as retentate. The green path represents the portion of the sample that passes through the membrane and exits separately as permeate.
EVlution™ works with a range of hollow fibre filters with different pore sizes for different applications which include:
- Recovering proteins, antibodies, and EVs from cell culture media.
- Depletion of FBS-associated EVs
- Concentrating and purifying proteins, peptides, and nucleic acids.
- Buffer exchange (diafiltration)
Features and Benefits
Key Features and Benefits of the EVlution™ system |
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Excellent EV/exosome yield |
Compact & adaptable system |
Cost effective instrument |
12 pore sizes available |
Minimal hands on time |
Comparison of Common EV Concentration Methods |
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Ultra- centrifugation |
Precipitation |
EVlution TFF |
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Price |
High | Low | Moderate |
| Purity | Moderate | Low |
High |
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Throughput |
Low | High | High |
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Yield |
Moderate | Moderate | High |
Product Guide
The adaptable design of EVlution™ supports a wide range of hollow fiber filter configurations, making it a flexible solution for diverse laboratory-scale applications. With an intuitive interface and streamlined operation, EVlution™ simplifies complex filtration processes while delivering reliable performance. The polyethersulfone hollow fibre TFF cartridges are available in 12 different pore sizes, allowing you to customize your EVlution system to suit your research needs. Our 500 kDa cartridge is the optimal pore size for exosome concentration to generate small volumes with high exosome yield. The EVlution system can be used in conjuction with our Exo-spin™ Purification columns. The system is easy to clean and the filters are reusable up to 20 times, providing you with a system to advance your exosome workflow.