EVlution TFF Resources
Highlighted Publications on TFF for Extracellular Vesicle Isolation
EVlution™ is a new instrument and peer-reviewed publications citing the system itself are forthcoming. The publications below establish tangential flow filtration as the gold-standard method for scalable, gentle, and reproducible concentration of extracellular vesicles, and provide the methodological foundation on which EVlution™ was designed.
Foundational TFF for EV Literature |
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Study |
EV Source |
Key Finding |
| Busatto et al. (2018) Cells | Conditioned media, patient lipoaspirate | TFF gives higher yield, fewer sub-15 nm aggregates, and improved batch-to-batch consistency versus ultracentrifugation, at half the processing time. |
| Heinemann et al. (2014) J Chromatogr A | Conditioned media (large volume) | Benchmark protocol combining TFF concentration with downstream chromatography for high-purity EV preparations. |
| Corso et al. (2017) Sci Rep | Cell culture supernatant | Combined TFF and bind-elute size-exclusion chromatography for scalable isolation of intact, functional EVs. |
| Welton et al. (2015) J Extracell Vesicles | Urine | Demonstrated TFF as an effective concentration method for urinary EVs prior to proteomic analysis. |
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Customer publications using EVlution™ will be added here as they appear. To request your work be highlighted, please contact us.
EVlution™ Compatible Workflows
EVlution™ is a benchtop tangential flow filtration system designed for size-based concentration and buffer exchange of extracellular vesicles and other nanoparticles from a wide range of biological starting materials. The table below summarises validated and recommended workflows. Click on the downstream link for the recommended polishing and characterisation steps.
Recommended starting materials and workflows |
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Cell culture supernatants |
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Starting Material |
Typical Volume |
Application |
Recommended Downstream |
| Adherent culture (flasks, multi-layer) | 250 mL - 5 L | EV concentration, buffer exchange | Exo-spin SEC |
| Suspension culture / bioreactor | 500 mL - 10 L | High-volume EV concentration | Exo-spin SEC |
| MSC and stem cell conditioned media | 250 mL - 5 L | Therapeutic EV concentration | Exo-spin SEC + NTA |
Biological fluids |
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| Urine | 50 mL - 1 L | Biomarker discovery, liquid biopsy | Exo-spin SEC |
| Plasma / serum (diluted) | 100 mL - 1 L | Clinical EV research | Exo-spin SEC |
| Milk and dairy fluids | 100 mL - 2 L | Food science, oral delivery research | Exo-spin SEC |
| Saliva | 10 mL - 250 mL | Oral biomarker research | Exo-spin SEC |
Other applications |
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| Plant-derived EVs / exosome-like nanoparticles | 100 mL - 5 L | Plant EV concentration | Exo-spin SEC |
| Bacterial outer membrane vesicles (OMVs) | 250 mL - 5 L | Vaccine and microbiome research | Custom workflow |
| Viral particles and lentiviral vectors | 250 mL - 5 L | Viral concentration and buffer exchange | Custom workflow |
| Recombinant proteins and antibodies | 250 mL - 5 L | Protein concentration, diafiltration | Application-specific |
Working volumes are indicative and depend on cartridge selection, target concentration factor, and starting material characteristics. Please contact us for guidance on your specific application.
Choosing a TFF Method for Your Sample
The table below summarises how tangential flow filtration compares with alternative EV isolation methods, to help you decide whether EVlution™ TFF is the right fit for your workflow.
EV isolation method comparison |
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Method |
Scalability |
EV Integrity |
Processing Time |
Typical Use |
| Tangential Flow Filtration (EVlution™) | Excellent (250 mL to 10 L+) | High (gentle, low shear) | 1 to 3 hours | Concentration, diafiltration, large volumes |
| Ultracentrifugation | Poor (rotor volume limited) | Moderate (mechanical stress) | 4 to 18 hours | Pellet-based isolation, small volumes |
| Size Exclusion Chromatography (Exo-spin) | Limited per column | High (gentle) | 30 to 60 minutes | Polishing, protein removal |
| Precipitation (PEG-based) | Moderate | Low (co-precipitates contaminants) | Overnight | Crude enrichment |
| Immunoaffinity capture | Poor | Variable (elution conditions) | 2 to 4 hours | EV subpopulation isolation |
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For most workflows, EVlution™ TFF is the recommended first step for sample concentration, followed by Exo-spin SEC for downstream polishing.
EVlution™ TFF System: Product Overview
Setting up and using EVlution
Introducing the EVlution™ TFF system: an affordable, benchtop tangential flow filtration platform designed specifically for extracellular vesicle research. EVlution™ brings the power of TFF, traditionally reserved for industrial bioprocessing, to the laboratory bench, enabling researchers to concentrate and purify EVs from large volumes of conditioned media, urine, plasma, and other biological fluids in a single, gentle, reproducible workflow.
For technical support, please get in touch with us by email at [email protected].
Appendix: Resource Summaries
1. EVlution™ TFF User Guide
The EVlution™ User Guide is the complete reference manual for setting up, operating, cleaning, and maintaining the EVlution™ TFF system. It covers unboxing and installation, fluid path assembly, pump control and flow rate selection, transmembrane pressure (TMP) monitoring, concentration and diafiltration workflows, cleaning-in-place procedures, cartridge storage, and troubleshooting common operational issues. This guide is essential reading for all EVlution™ users and is referenced throughout the protocol documents.