Exo-spin Purification
Economical and Reliable EV isolation. Trusted for more than ten years in hundreds of EV studies
*First-time users within a department
Simple Principles and Workflow
Size exclusion chromatography (SEC) separates particles in solution based on size. An SEC column is packed with a resin bed containing stable polymeric spherical beads, each of which has nano-scale pores across its surface. The beads leave gaps after packing into the column, the size of which depends on the diameter of the beads. When a solution containing exosomes is added to the top of the resin bed, the smallest particles, including free-proteins, become trapped in the nano-sized pores, slowing their progress, whereas the larger exosomes pass around the outside of the beads and elute first. The very largest particles, beyond the size of the gaps between the beads, are also slowed or stopped completely. The resin used in Exo-spin columns has been chosen to specifically allow primary elution of particles in the range 30nm - 250nm.
Simple, easy-to-use protocols for reproducible results.
- The sample is spun to remove cells and (optional step) precipitated to concentrate exosomes.
- The column closures are removed
- The column is equilibrated
- The sample is added
- Purified exosomes are eluted
Features and Benefits
Key Features and Benefits of the Exo-spin™ system |
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Excellent yields and high levels of purity |
No ultracentrifugation |
Simple and reliable |
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Exosomes with low levels of protein and rRNA contamination |
The protocol provides consistent results every time |
Isolate intact whole exosomes for functional studies |
Comparison of EV Common Isolation Methods |
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Exo-spin + Precipitation |
Exo-spin |
Ultra- centrifugation |
Precipitation |
Affinity-capture |
Lateral flow |
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Price |
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Capacity |
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| Purity | +++ | +++ | +++ | + | +++ |
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| Subtype selection | - | - | - | - | + |
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Throughput |
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Yield |
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A comparison of commonly used EV isolation techniques. Exo-spin provides excellent cost/performance
Product Selection Guide
Exo-spin™ purification columns utilise trusted size exclusion chromatography (SEC) technology. The columns are available in a variety of resin volumes, bed surface areas and bed lengths. The shorter bed lengths provide more rapid protocols and lower costs. For most downstream applications, the 99% purity provided by Exo-spin™ mini, the shortest Exo-spin™ column, provides excellent results.
Sample volumes from 0.1 ml to 1.0 ml may be applied directly to columns. Larger sample volumes can be concentrated before application using Exo-spin™ buffer or added iteratively*.
* Large samples can be broken down into smaller aliquots. The column is flushed between aliquots to remove non-exosomal components of previous samples. Exo-spin™ columns can each be used up to 5 times either for iterative loading to purify a larger volume of a sample, or using different samples. See User Guide for further details.
Exo-spin™ is available in various formats.
- EX03 Exo-spin™ mini
- EX04 Exo-spin™ midi
- EX05 Exo-spin™ mini HD
- EX07 Exo-spin™ 96 (mini in a 96-well format)
or for handling larger volumes of dilute samples (e.g. saliva, urine, culture media) Exo-spin™ is provided with precipitant buffer with columns
- EX01 Exo-spin™ mini - for cell culture media, urine, and other low-density exosome sources
- EX02 Exo-spin™ mini blood - for plasma and sera
Additional Exo-spin™ precipitant buffer is also available separately
- EX06 Exo-spin™ Precipitant Buffer
The tables below guide the selection of the right Exo-spin™ kit to purify exosomes from your sample. If you need help choosing the right product, please get in touch or use the online chat function (at the bottom right of this page).
Low exosome-content biofluids (up to 1x109/ml) including cell culture media, saliva, cerebrospinal fluid, urine requiring concentration before application to the column |
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Sample Volume* |
isolation method |
Exo-spin Kit |
column bed length (purity)# |
| < 1ml to 250 ml |
concentration + SEC |
EX01, EX07 |
1.3 cm (99.0%) |
| < 1 ml to 375 ml |
concentration + SEC |
EX05 |
6.35 cm (99.8%) |
| 75 ml to 500 ml | concentration + SEC | EX04 | 2.15 cm (99.5%) |
Plasma and Sera - samples are usually added directly to the Exo-spin column, but may be concentrated prior to application. |
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Sample volume added to column* |
isolation method |
Exo-spin Kit |
column bed length (purity)# |
| 100 µl |
SEC |
EX03 (mini), EX07 (96) |
1.3 cm (99.0%) |
| 150 µl |
SEC |
EX05 (mini-HD) | 6.35 cm (99.8%) |
| 100 µl | concentration + SEC | EX02 (Mini), EX07 (96) | 1.3 cm (99.0%) |
| 1 ml | SEC | EX04 (Midi) | 2.15 cm (99.5%) |
*Note: Samples may be concentrated before adding to the column using Exo-spin buffer or ultrafiltration. Each column may be loaded up to five times. The iterative loading of a single sample increases the maximum sample size to the volume shown above.
#Note: A longer bed length improves the resolution, removal of free protein, and purity of exosomes. The purity levels indicated are typical when following the protocol provided.
All CellGS products are supported by experienced technical specialists. If you need any advice on selecting or using Exo-spin, or any other product, please contact our support team at [email protected], or use the online chat function (bottom right of this page)