Exo-spin Purification
Simple, easy-to-use products and protocols for reproducible results. Protocol overview. (1) The sample is spun to remove cells and (optional step) precipitated to concentrate exosomes. (2) The column closures are removed (3) the column is equilibrated (4) the sample is added (5) purified exosomes are eluted
Exo-spin™ enables the isolation of exosomes from a range of sample inputs for varied downstream applications.
Exo-spin™ purification columns utilize trusted size exclusion chromatography (SEC) technology. The columns are available in a variety of resin volumes, bed surface areas and lengths. The shorter bed lengths provide more rapid protocols and lower costs. For most downstream applications, the 99% purity provided by Exo-spin™ mini, the shortest Exo-spin™ column, provides excellent results.
Sample volumes from 0.1 ml to 1.0 ml may be applied directly to columns. Larger sample volumes can be concentrated before application using Exo-spin™ buffer or added iteratively*.
* Large samples can be broken down into smaller aliquots. The column is flushed between aliquots to remove non-exosomal components of previous samples. Exo-spin™ columns can each be used up to 5 times either for iterative loading to purify a larger volume of a sample, or using different samples. See User Guide for further details.
Click on the column image to see product details
Exo-spin™ is available as columns.
- EX03 Exo-spin™ mini
- EX04 Exo-spin™ midi
- EX05 Exo-spin™ mini HD
- EX07 Exo-spin™ 96 (mini in a 96 well format)
or for handling larger volumes of dilute samples (e.g. saliva, urine, culture media) Exo-spin™ is provided with precipitant buffer with columns
- EX01 Exo-spin™ mini - for cell culture media, urine, and other low-density exosome sources
- EX02 Exo-spin™ mini blood - for plasma and sera
Additional Exo-spin™ precipitant buffer is also available separately
- EX06 Exo-spin™ Precipitant Buffer
Key Features of the Exo-spin™ system |
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Excellent yields and high levels of purity |
No ultracentrifugation |
Simple and reliable |
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Exosomes with low levels of protein and rRNA contamination |
The protocol provides consistent results every time |
Isolate intact whole exosomes for functional studies |
A comparison of commonly used EV isolation techniques. Exo-spin provides excellent cost/performance
Product selection
The tables below offer a guide to selecting the right Exo-spin™ kit to purify exosomes from your sample. If you need help choosing the right product, please get in touch or use the online chat function (bottom right of this page).
Low exosome-content biofluids (up to 1x109/ml) including cell culture media, saliva, cerebrospinal fluid, urine requiring concentration before application to the column |
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Sample Volume* |
isolation method |
Exo-spin Kit |
column bed length (purity)# |
| < 1ml to 250 ml |
concentration + SEC |
EX01, EX07 |
1.3 cm (99.0%) |
| < 1 ml to 375 ml |
concentration + SEC |
EX05 |
6.35 cm (99.8%) |
| 75 ml to 500 ml | concentration + SEC | EX04 | 2.15 cm (99.5%) |
Plasma and Sera - usually added directly to the column, but may be concentrated prior to application. |
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Sample volume added to column* |
isolation method |
Exo-spin Kit |
column bed length (purity)# |
| 100 µl |
SEC |
EX03 (mini), EX07 (96) |
1.3 cm (99.0%) |
| 150 µl |
SEC |
EX05 (mini-HD) | 6.35 cm (99.8%) |
| 100 µl | concentration + SEC | EX02 (Mini), EX07 (96) | 1.3 cm (99.0%) |
| 1 ml | SEC | EX04 (Midi) | 2.15 cm (99.5%) |
*Note: Samples may be concentrated before adding to the column using Exo-spin buffer or ultrafiltration. Each column may be loaded up to five times. The iterative loading of a single sample increases the maximum sample size to the volume shown above.
#Note: A longer bed length improves the resolution, removal of free protein, and purity of exosomes. The purity levels indicated are typical when following the protocol provided.
Exosome purification using size exclusion chromatography (SEC)
SEC separates particles in solution based on their size. An SEC column is packed with resin containing stable polymeric spherical beads, each of which has large numbers of nano-scale pores across its surface. The beads leave gaps after packing, the size of which depends on the diameter of the beads. When a solution containing exosomes is added to the top of the resin bed, the smallest particles, including free-proteins, become trapped in the nano-sized pores, slowing their progress, whereas the larger exosomes pass around the outside of the beads and elute first. The very largest particles, beyond the size of the gaps between the beads, are also slowed or stopped completely. The resin used in Exo-spin columns has been chosen to specifically allow primary elution of particles in the range 30nm - 250nm. This allows the isolation of exosomes from particles of other sizes.
Read our support literature
All CellGS products are supported by technical specialists. If you need any advice on selecting or using Exo-spin, or any other product, please contact our support team at [email protected] or use the online chat function (bottom right of this page)
Whatever the sample and whatever the application, there is an Exo-spin™ product for your needs.