Exo-spin Purification
Exo-spin™ is a modular system that adapts to your changing research needs by providng purification of exosomes from a wide variety of sample inputs for a range of downstream applications.
Exo-spin™ uses size exclusion chromatography (SEC) columns. A proprietary precipitant buffer may be used to concentrate exosomes before purification on the column. This precipitation is optional for blood, but for other exosome sources, including urine and cell culture media, the samples need concentrating prior to application. Alternative concentrating technologies, such as filtration, may also be used.
Exo-spin™ columns are available with a variety of resin volumes and bed lengths. The shorter bed lengths provide more rapid protocols and lower costs. For the majority of downstream applications, the 99% purity provided by Exo-spin™ mini, the shortest Exo-spin™ column, provides excellent results.
Exo-spin™ is available either as individual columns
- EX03 Exo-spin™ mini
- EX04 Exo-spin™ midi
- EX05 Exo-spin™ mini HD
- EX07 Exo-spin™ 96 (mini in a 96 well format)
or as kits that provide Exo-spin™ precipitant buffer with columns
- EX01 Exo-spin™ mini - for cell culture media, urine, and other low-density exosome sources
- EX02 Exo-spin™ mini blood - for plasma and sera
Additional Exo-spin™ precipitant buffer (EX06) may be purchased separately.
Exo-spin™ columns can each be used up to 5 times either for iterative loading to purify a larger volume of a sample, or using different samples.
Key Features of the Exo-spin™ system |
||
|
Excellent yields and high levels of purity |
No ultracentrifugation |
Simple and reliable |
|
Exosomes with low levels of protein and rRNA contamination |
The protocol provides consistent results every time |
Isolate intact whole exosomes for functional studies |
Product selection
To get started, the tables below provide a guide to selecting the right Exo-spin™ kit to purify exosomes from your sample. If you need help making your selection, please get in touch or simply use the online chat function (bottom right of this page).
Low exosome-content biofluids (up to 1x109/ml)including cell culture media, saliva, cerebrospinal fluid, urine |
|||
|
Sample Volume* |
isolation method |
Exo-spin Kit |
column bed length (purity)# |
| < 1ml to 250 ml |
concentration + SEC |
EX01, EX07 |
1.3 cm (99.0%) |
| < 1 ml to 375 ml |
concentration + SEC |
EX05 |
6.35 cm (99.8%) |
| 75 ml to 500 ml | concentration + SEC | EX04 | 2.15 cm (99.5%) |
Plasma and Sera |
|||
|
Sample Volume* |
isolation method |
Exo-spin Kit |
column bed length (purity)# |
| 100 - 500 µl |
SEC |
EX03 , EX07 |
1.3 cm (99.0%) |
| 150 - 750 µl |
SEC |
EX05 | 6.35 cm (99.8%) |
| 100 µl - 2500 µl | concentration + SEC | EX02, EX07 | 1.3 cm (99.0%) |
| 1 - 5 ml | SEC | EX04 | 2.15 cm (99.5%) |
*Note Columns may be loaded up to five times. Iterative loading of a single sample increases the maximum sample size to the volume shown above.
#Note longer bed length improves the resolution, removal of free protein, and purity of exosomes. Purity levels achieved are typical following the protocol provided.
Exosome purification using size exclusion chromatography (SEC)
SEC separates particles in solution based on their size. An SEC column is packed with resin containing stable polymeric spherical beads, each of which has large numbers of nano-scale pores across its surface. The beads leave gaps after packing, the size of which depends on the diameter of the beads. When a solution containing exosomes is added to the top of the resin bed, the smallest particles, including free-proteins, become trapped in the nano-sized pores, slowing their progress, whereas the larger exosomes pass around the outside of the beads and elute first. The very largest particles, beyond the size of the gaps between the beads, are also slowed or stopped completely. The resin used in Exo-spin columns has been chosen to specifically allow primary elution of particles in the range 30nm - 250nm. This allows the isolation of exosomes from particles of other sizes.
Read our support literature
All CellGS products are supported by technical specialists. If you need any advice on selecting or using Exo-spin, or any other product, please contact our support team at tech@cellgs.com or simply use the online chat function (bottom right of this page)
Whatever the sample and whatever the application, there is an Exo-spin™ product for your needs.