More EVs. Less effort. Lower cost.
The EVlution™ TFF System delivers high-yield, high-purity EV purification from up to 3 litres of starting material - in just 90 minutes. Unlike ultracentrifugation, EVlution™ is affordable, easy to use, and requires minimal hands-on time, freeing you to focus on what matters most: your research.
Its innovative Switch Flow™ technology reduces retentate volume to under 10 mL, maximising recovery while minimising loss. The compact, modular design supports 12 interchangeable polyethersulfone hollow fibre cartridge sizes, including the widely used 300 kDa and 500 kDa options optimised for EV concentration. Filters are reusable up to 20 times, keeping running costs low. EVlution™ pairs seamlessly with Exo-spin™ purification columns for a complete, end-to-end EV workflow.
Whether you're concentrating EVs, depleting FBS-associated particles, or performing buffer exchange, EVlution™ has you covered.
Matrigel is widely used to support inoculated cancer cells as they establish tumours in mouse cancer models. But the poorly defined nature of Matrigel, and the high levels of cytokines it contains, may be confounding the interpretation of data.
A systematic look at how to optimize TFF for EV purification: what each parameter controls, how to set it, and how to confirm that the resulting preparation meets the quality standards that downstream work, whether cargo analysis, functional assay or therapeutic manufacture, demands.
Three analytical layers dominate EV analysis. Proteomics catalogues the proteins, transcriptomics profiles the RNA, and lipidomics maps the membrane lipids. Together they form the core of EV cargo analysis.
How neuron-derived extracellular vesicles (NDEVs) carry amyloid-beta, tau and alpha-synuclein between neurons and provide blood biomarkers for Alzheimer's, Parkinson's, ALS and FTD, plus the growth factor and EV isolation tools that support the work.
This article surveys the growth factors that underpin organoid culture, why they matter, and how their delivery shapes organoid quality, reproducibility and cost. It explains the roles of the core signalling families, why stem-cell niches depend on tightly regulated factor gradients, and how the practical challenges of growth factor instability and expense are driving interest in sustained-release approaches.
How polyhedrin functions as a viral survival strategy, and how the PODS (Polyhedrin Delivery System) mechanism harnesses polyhedrin's crystalline architecture to encapsulate growth factors and control their release over days to weeks.
Three single-particle methods dominate the modern EV characterisation toolkit: nanoparticle tracking analysis (NTA), tunable resistive pulse sensing (TRPS) and microfluidic resistive pulse sensing (MRPS). Which should you choose? Or do you need them all?
Several methods have emerged that dominate the field of extracellular vesicle (EV) isolation. Beyond these, many interesting alternatives are on the horizon.
