Tangential flow filtration (aka Cross flow filtrati is the method of choice when sample volume, yield and scalability are the primary requirements. It is the only isolation approach that can process litre-scale conditioned media volumes in a single continuous run without filter fouling, and it simultaneously concentrates EVs to a small, analytically ready output volume.

The isolation of extracellular vesicles (EVs) and exosomes is one of the most critical and variable steps in any EV research workflow. The most commonly used non-affinity isolation methods are ultracentrifugation (UC), size exclusion chromatography (SEC), and tangential flow filtration (TFF). The method you choose directly determines the purity, yield and integrity of your isolated vesicles, and has downstream consequences for every subsequent characterisation step, including nanoparticle tracking analysis (NTA), western blot and functional assays

For decades, researchers have been searching for a reliable, clinically translatable way around this formidable BBB obstacle. Researchers in China may now have found one.

Theories can become pervasive without any real evidence. EVs, once thought as trash containers, are not alone: Stress causes stomach ulcers. Consensus had non-coding DNA as junk. We look at past examples and current science that, perhaps, should not be counted as settled.

By analysing tumour-derived material circulating in blood, urine, or other body fluids, liquid biopsies offer a minimally invasive window into cancer biology in real time. Among the various analytes being explored, including circulating tumour cells (CTCs) and cell-free DNA (cfDNA), extracellular vesicles (EVs) are emerging as particularly compelling biomarker candidates.

A landmark study defines the protein and lipid signatures that distinguish authentic small extracellular vesicles in human plasma.

How TFF works, filter formats compared, and why EVlution belongs on your bench

EVlution, a new affordable bench top Tangential Flow Filtration (TFF) system launches today. It makes scalable, reproducible extracellular vesicle purification accessible to more laboratories, accelerating innovation in EV research and translational development.