Purified Exosomes
Tools for discovery
Exosomes are complex variable structures which require basic characterization as part of any study. Guidance on the minimal level of characterization that should be performed before a study can be published has been provided by the exosome community. Cell Guidance Systems has developed a range of solutions based on exosome composition and characteristics. These include: exosome marker antibodies, ExoLISA™ detection assay, Instant Exosomes™ and we also offer an NTA size profiling service.
Instant Exosomes™
Instant Exosomes™ are a range of high-quality and well characterized freeze-dried exosome samples from human cancer cell lines. Exosomes are isolated following a combination of precipitation and size exclusion chromatography (SEC). The exosome samples are characterized for overall protein content, using a BCA assay, expression of commonly expressed exosomal markers (CD9, CD63 and CD81) using the ExoLISA™ exosome detection assay and, particle concentration and size distribution by nanoparticle tracking analysis (NTA) using a Zetaview® instrument from Particle Metrix GmbH.
Instant Exosomes™ are isolated and available from the following human cancer cell lines and stem cells:
- EX301 Instant Exosomes™ from COLO205, a human colon carcinoma cell line
- EX302 Instant Exosomes™ from LnCAP, a human prostate carcinoma cell line
- EX303 Instant Exosomes™ from A375, a human malignant melanoma cell line
- EX304 Instant Exosomes™ from Mesenchymal Stem Cells (MSCs)