


Tools for discovery
Exosomes are complex variable structures which require basic characterization as part of any study. Guidance on the minimal level of characterization that should be performed before a study can be published has been provided by the exosome community. Cell Guidance Systems has developed a range of solutions based on exosome composition and characteristics. These include: exosome marker antibodies, ExoLISA™ detection assay, Instant Exosomes™ and we also offer an NTA size profiling service.
Instant Exosomes™
Instant Exosomes™ are a range of high-quality and well characterized freeze-dried exosome samples from human cancer cell lines. Exosomes are isolated following a combination of precipitation and size exclusion chromatography (SEC). The exosome samples are characterized for overall protein content, using a BCA assay, expression of commonly expressed exosomal markers (CD9, CD63 and CD81) using the ExoLISA™ exosome detection assay and, particle concentration and size distribution by nanoparticle tracking analysis (NTA) using a Zetaview® instrument from Particle Metrix GmbH.
Instant Exosomes™ are isolated and available from the following human cancer cell lines and stem cells:
- EX301 Instant Exosomes™ from COLO205, a human colon carcinoma cell line
- EX302 Instant Exosomes™ from LnCAP, a human prostate carcinoma cell line
- EX303 Instant Exosomes™ from A375, a human malignant melanoma cell line
- EX304 Instant Exosomes™ from Mesenchymal Stem Cells (MSCs)