EVlution 260cm2 Cartridges
Product description
EVlution™ 260 cm² replacement cartridges are designed for use with the EVlution™ TFF System, enabling high-yield concentration and purification of EVs, exosomes, proteins, and nucleic acids from up to 3 litres of starting material.
Cartridges are available in 10 pore sizes ranging from 5 kDa to 1000 kDa; each cartridge uses precision polyethersulfone hollow fibre membranes to deliver consistent, reproducible results. Cartridges are reusable up to 20 times, making them a cost-effective choice for high-throughput research workflows. For EV concentration, 300 kDa and 500 kDa cartridges are most widely recommended.
| Product Details | |
|---|---|
| Storage temperature |
Room temperature |
| Processable Volume |
Up to 3 litres |
| Cartridge area |
260 cm2 |
| Cartridge pore size |
Available sizes include 1, 3, 5, 8. 10, 30, 50, 100, 300, 500, 750, 1000 kDa. |
EV purification
Sample to EV workflow
EVlution is part of a complete workflow for the isolation of EVs. After EVlution, additional EV purification, if needed, can be achieved with Exo-spin SEC columns
EV concentration and removal of contaminating proteins
EVlution TFF enables the retention of EVs within your samples when concentrating large volumes of biological fluids.
EVlution TFF significantly reduces the concentration of free proteins within your sample when concentrating large volumes of biological fluids.
Hollow Fibre Filter Applications by Pore Size |
|||
| Pore Size (kDa) | Approx. Pore Diameter (nm) | Retained Molecules | Typical Applications |
| 1 | ~1 | Peptides, proteins, nucleic acids, EVs | Concentration of small peptides and proteins from dilute solutions |
| 3 | ~2 | Small proteins, nucleic acids, EVs | Concentration and purification of small proteins; buffer exchange |
| 5 | ~3 | Small proteins, nucleic acids, EVs | Protein concentration; removal of salts and small molecules |
| 8 | ~4 | Proteins, nucleic acids, EVs | Protein purification; diafiltration |
| 10 | ~5 | Proteins, cytokines, nucleic acids, EVs | Cytokine and growth factor concentration; buffer exchange |
| 30 | ~8 | Large proteins, antibody fragments, EVs | Antibody fragment purification; depletion of small proteins |
| 50 | ~12 | Albumin, large proteins, EVs | Albumin removal; large protein concentration |
| 100 | ~18 | Large protein complexes, EVs | Concentration of large protein complexes; pre-filtration for EV isolation |
| 300 | ~25 | EVs / Exosomes, large complexes | EV concentration and purification; FBS-EV depletion |
| 500 | ~45 | EVs / Exosomes, microvesicles | EV concentration and purification; FBS-EV depletion |
| 750 | ~65 | EVs, microvesicles, large particles | Microvesicle isolation; removal of smaller EVs and proteins |
| 1000 | ~100 | Large EVs, microvesicles, apoptotic bodies | Large particle concentration; apoptotic body isolation |
Most widely used pore sizes for EV concentration
Product documents
A cartridge specific Certificate of Analysis (CoA) is available on request.
