EVlution TFF System
Product description
EVlution™ is an affordable, innovative and compact tangetial flow filtration (TFF) platform that provides efficient size-dependent concentration and purification of EVs and other molecules. EVlution has the unique ability to generate sub-10 ml samples from large starting volumnes
TFF provides an effective method of size-based separation of EVs from other biomolecules, whilst minimizing membrane fouling. The characteristics of TFF design makes it an excellent choice for workflows at the lab scale. The adaptable design of EVlution™ supports a wide range of hollow fiber filter configurations, making it a flexible solution for diverse laboratory-scale applications. With an intuitive interface and streamlined operation, EVlution™ simplifies complex filtration processes while delivering reliable performance. EVlution™ incorporates an ingenious circulated to reversible flow switching mechanism which allows sample volume reduction to below 10 ml.
EVlutionTM System includes everything required to perform TFF isolation:
- The EVlution stand
- A 94cm2 cartridge (300 or 500 kDa only)
- The EVlution pump
- 1 set of syringes
- 1 set of tubing and valves
- Accessories (clips and pressure gauge housing)
Note: Duran bottles are NOT included.
For further details, contact us at [email protected]
| Product Details | |
|---|---|
| Storage temperature |
Room temperature |
| Processable Volume |
Up to 1 litre |
| Cartridge area |
94 cm2 |
| Cartridge pore size |
Choose from 300 or 500 kDa |
| System weight |
2.7 kg |
| Footprint |
21 cm x 26.5 cm |
Product attributes
Purpose-built for EVs with hollow fibre technology. EVlution uses hollow fibre filter cartridges to provide the low-shear, laminar flow environment that extracellular vesicles need. This preserves vesicle integrity and biological function, giving you confidence that what you isolate faithfully represents what was in your sample.
Innovative Switch Flow™ for the smallest output volumes. The ability to switch from circulated to reversible retentate flow enables a final retentate volume below 10 ml
Genuinely affordable. EVlution is accessible to individual research groups, core facilities and teaching laboratories.
Compact benchtop footprint. With dimensions of just 21 × 26.5 cm and a weight of 2.7 kg, EVlution takes up less bench space than a standard laboratory balance. It requires no dedicated infrastructure — just plug in and go.
Flexible filter configurations. The adaptable design supports a range of hollow fibre cartridges with 300 kDa or 500 kDa MWCO options, along with different cartridge sizes (94 cm² and 260 cm² membrane area). This allows you to match the system to your specific application, whether you are processing small clinical samples or larger volumes of conditioned media up to one litre.
Simple, intuitive operation. EVlution has been designed for ease of use. Researchers can set up and run a TFF process without extensive training or bioprocessing expertise. The streamlined workflow lowers the barrier to adoption and reduces the risk of operator error.
Proven protein removal performance. Data from Cell Guidance Systems demonstrates that EVlution significantly reduces the concentration of free proteins in the retentate during EV concentration, as shown by BCA protein assay, while NTA particle tracking confirms efficient recovery of EV particles. This dual evidence of concentration and purification in a single step saves time and reduces sample handling.
Part of a complete EV workflow. Cell Guidance Systems offers a comprehensive EV product portfolio including Exo-spin™ SEC columns, ExoLISA™ assays, validated EV antibodies, purified reference exosomes, and NTA profiling services. EVlution integrates seamlessly as the front-end concentration and initial purification step in a multi-stage workflow — for example, TFF followed by Exo-spin SEC for high-purity exosome isolation.
Backed by EV expertise. Cell Guidance Systems is a specialist in extracellular vesicle research tools. When you purchase EVlution, you gain access to a team with deep knowledge of EV biology and purification who can advise on protocol optimisation and troubleshooting.
Product data
EV concentration and removal of contaminating proteins
EVlution TFF enables the retention of EVs within your samples when concentrating large volumes of biological fluids.
EVlution TFF significantly reduces the concentration of free proteins within your sample when concentrating large volumes of biological fluids.
Video
Setting up and using EVlution
