Orangu™ Cell Counting Solution

Code Description Price Qty
OR01-500 Orangu™, 500 tests £89.00
OR01-1000 Orangu™, 1000 tests £139.00
OR01-3000 Orangu™, 3000 tests £298.00

Overview

Orangu™ is a non-cytotoxic, sensitive, colorimetric assay for the determination of cell viability in cell proliferation and cytotoxicity assays. Orangu™ utilizes WST-8, a highly water-soluble tetrazolium salt that is reduced by dehydrogenase activities in viable cells, to give an orange-coloured formazan dye. The amount of dye formed is directly proportional to the number of living cells. Increasing incubation periods or number of cells will lead to an increase in the amount of orange dye. Simple! 

 

Three simple steps to use Orangu™:

Step 1   Step 2   Step 3 

 

Step 1: Add Orangu™ to your cells,

Step 2: Incubate (typically 1 - 4 hours),

Step 3: Read absorbance at 450 nm in a standard plate reader.

 

Product Data

Orangu™ utilizes WST-8, a highly water-soluble tetrazolium salt that is reduced by dehydrogenase activities in viable cells to give an orange-coloured formazan dye.

Orangu™ demonstrates higher levels of sensitivity than commonly used alternatives MTT, XTT, MTS or WST-1.

 

Generation of a standard curve using HeLa cells.

Citations

Lutter AH, Scholka J, Richter H, Anderer U. Applying XTT, WST-1, and WST-8 to human chondrocytes: A comparison of membrane-impermeable tetrazolium salts in 2D and 3D cultures. (2017) Clin Hemorheol Microcirc doi: 10.3233/CH-179213