Media & Matrices Exosomes Matrigen Softwell Growth Factors Small Molecules Gene Knock-Up Karyotype Service

Recombinant Mouse SCF Protein

   

Stem Cell Factor

 

Recombinant mouse Stem Cell Factor (SCF) is involved in survival, differentiation and activation of myeloid, erythroid, lymphoid, mast cells, eosinophils and other progenitor cells.  mSCF signals through its receptor, tyrosine kinase SCF R/c-kit. It is a single, non-glycosylated polypeptide chain.

 

Recombinant Mouse SCF is fully biologically active when compared to standard. The ED50, as determined by the dose-dependent stimulation of Human TF-1 cells, is 5.5 ng/ml.

         
164 αα 18.3 kDa P20826
>98% E. coli -20oC


Physical Appearance

Sterile filtered white lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a concentrated (1 mg/ml) solution in water containing 10 mM Sodium phosphate buffer pH=6.5.

Reconstitution

Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.

Stability

Lyophilized product is very stable at -20°C. Reconstituted material should be aliquoted and frozen at -20°C. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.

Protein Content as determined by

UV spectroscopy at 280 nm. Analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.

Endotoxin Level

Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.

Alternative Names

Stem cell factor, SCF

AA Sequence

KEICGNPVTD NVKDITKLVA NLPNDYMITL NYVAGMDVLP SHCWLRDMVI QLSLSLTTLL DKFSNISEGL SNYSIIDKLG KIVDDLVLCM EENAPKNIKE SPKRPETRSF TPEEFFSIFN RSIDAFKDFM VASDTSDCVL SSTLGPEKDS RVSVTKPFML PPVA

 

RESEARCH USE ONLY