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Recombinant Mouse SCF Protein

   

Stem Cell Factor

 

Stem Cell Factor (SCF) is a cytokine made by fibroblasts and endothelial cells. SCF binds to the receptor known as c-Kit (CD117) and is thought to play a critical role in the maintenance or survival of hematopoietic stem cells. Human SCF shows no activity on murine cells, but murine and rat SCF are active on human cells. Mouse SCF is a single, non-glycosylated polypeptide chain.

 

Recombinant Mouse SCF is fully biologically active when compared to standard. The activity is determined by the dose-dependent proliferation of human TF-1 cells and is typically less than 10 ng/ml.

         
164 αα 18.3 kDa P20826
>98% E. coli -20oC


Physical Appearance

Sterile filtered white lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a concentrated (1 mg/ml) solution containing 10 mM Na3PO4, pH 6.5.

Reconstitution

Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.

Stability

Lyophilized product is very stable at -20°C. Reconstituted material should be aliquoted and frozen at -20°C. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.

Protein Content as determined by

UV spectroscopy at 280 nm. Quantitation on SDS-PAGE against a known standard.

Endotoxin Level

Endotoxin level, as measured by LAL, is <0.01ng/ug or <0.1EU/ug.

Alternative Names

Stem cell factor, c-Kit Ligand, KL, Steel Factor, MGF

AA Sequence

KEICGNPVTD NVKDITKLVA NLPNDYMITL NYVAGMDVLP SHCWLRDMVI QLSLSLTTLL DKFSNISEGL SNYSIIDKLG KIVDDLVLCM EENAPKNIKE SPKRPETRSF TPEEFFSIFN RSIDAFKDFM VASDTSDCVL SSTLGPEKDS RVSVTKPFML PPVA

 

RESEARCH USE ONLY