Recombinant Mouse TNF-alpha Protein
Tumor Necrosis Factor-alpha
Tumor Necrosis Factor alpha (TNF-alpha) is an inflammatory cytokine secreted by macrophages, monocytes, neutrophils, T cells, NK-cells following their stimulation by bacterial LPS. TNF-alpha activates signals through two receptors: TNF-R1, which is expressed on most cell types, and TNF-R2, which is expressed mainly on immune cells. TNF-alpha can have many functions including to stimulate of phagocytosis in macrophages, to chemoattract neutrophils, to increase insulin resistance and to induce fever. Recombinant mouse TNF-alpha is a non-glycosylated protein.
TNF-alpha is fully biologically active when compared to standard. The ED50, as determined by the cytolysis of mouse L929 cells in the presence of Actinomycin D, is less then 100 pg/ml.
|156 αα||17.3 kDa||P06804
Sterile filtered white lyophilized (freeze-dried) powder.
Recombinant mouse TNF-alpha is lyophilized from 0.5 x PBS, pH 7.5.
Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Lyophilized product is very stable at -20°C. Reconstituted material should be aliquoted and frozen at -20°C. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.
Endotoxin level, as measured by LAL analysis, is less than 0.01 ng/ug or 0.1 EU/ug.
Protein Content determined by
UV spectroscopy at 280 nm. Analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.
Tumour necrosis factor alpha, TNFSF2, Cachectin, DIF, Necrosin, Cytotoxin, Cachexin, TNF
MLRSSSQNSS DKPVAHVVAN HQVEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLVY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK VNLLSAVKSP CPKDTPEGAE LKPWYEPIYL GGVFQLEKGD QLSAEVNLPK YLDFAESGQV YFGVIAL
RESEARCH USE ONLY