Recombinant Mouse TNF-alpha Protein
Tumor Necrosis Factor-alpha
Tumor Necrosis Factor alpha (TNF-α) is an inflammatory cytokine secreted by macrophages, monocytes, neutrophils, T-cells, NK-cells following their stimulation by bacterial LPS. TNF-αactivates signals through two receptors, TNF-R1, which is expressed on most cell types, and TNF-R2, which is expressed mainly on immune cells. TNF-α can have many functions including, to stimulate ofphagocytosis in macrophages, to chemoattract neutrophils, to increase insulin resistance and to induce fever. Recombinant mouse TNF-α is a non-glycosylated protein.
TNF-alpha is fully biologically active when compared to standard. The ED50 as determined by the cytolysis of mouse L929 cells in the presence of Actinomycin D is less then 0.05 ng/ml, corresponding to a specific activity of 9x108 IU/mg.
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Sterile filtered white lyophilized (freeze-dried) powder.
Each mg contains 50 mM Tris-HCL pH-8 and 20 mM NaCl.
Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Lyophilized product is very stable at -20°C. Reconstituted material should be aliquoted and frozen at -20°C. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.
Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.
Protein Content determined by
UV spectroscopy at 280 nm. Analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.
tumour necrosis factor, TNF
MLRSSSQNSS DKPVAHVVAN HQVEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLVY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK VNLLSAVKSP CPKDTPEGAE LKPWYEPIYL GGVFQLEKGD QLSAEVNLPK YLDFAESGQV YFGVIAL
RESEARCH USE ONLY