recombinant mouse TNF-alpha protein
Growth factors from Cell Guidance Systems are high purity suitable for the most demanding applications where biological activity is required.
Aliases
tumour necrosis factor, TNF
Description:
Recombinant mouse TNF-alpha produced in S.Lividians is a single, non- glycosylated, polypeptide chain containing 156 amino acids and having a molecular mass of 17301.32 Dalton.
Physical Appearance:
Sterile Filtered white lyophilized (freeze-dried) powder.
Source:
E. coli
Formulation:
Each mg contains 50mM Tris-HCL pH-8 and 20mM NaCL.
Reconstitution:
Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg’s/ml, which can be further diluted into other aqueous solutions.
Stability:
Lyophilized product is very stable at -20 degrees. Reconstituted material should be aliquoted and frozen at -20. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.
Purity Greater than 98% as determined by:
Analysis by RP-HPLC.
Reducing and non-reducing SDS- PAGE.
Endotoxin Level:
Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.
Protein Content determined by:
UV spectroscopy at 280 nm.
Analysis by RP-HPLC calibrated against a known standard.
Quantitation on SDS-PAGE against a known standard.
Biological Activity:
rmTNF-alpha is fully biologically active when compared to standard. The ED50 as determined by the cytolysis of mouse L929 cells in the presence of Actinomycin D is less then 0.05ng/ml, corresponding to a Specific Activity of 9x108 IU/mg.
AA Sequence:
MLRSSSQNSS DKPVAHVVAN HQVEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLVY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK VNLLSAVKSP CPKDTPEGAE LKPWYEPIYL GGVFQLEKGD QLSAEVNLPK YLDFAESGQV YFGVIAL
