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Recombinant Mouse TNF-alpha Protein

   
Tumor Necrosis Factor-alpha   Tumor Necrosis Factor alpha (TNF-α) is an inflammatory cytokine secreted by macrophages,monocytes, neutrophils, T-cells, NK-cells following their stimulation by bacterial LPS. TNF-αactivates signals through two receptors, TNF-R1, which is expressed on most cell types, and TNF-R2, which is expressed mainly on immune cells. TNF-α can have many functions including, to stimulate ofphagocytosis in macrophages, to chemoattract neutrophils, to increase insulin resistance and to induce fever.  Recombinant mouse TNF-α is a non-glycosylated protein.   rmTNF-alpha is fully biologically active when compared to standard. The ED50 as determined by the cytolysis of mouse L929 cells in the presence of Actinomycin D is less then 0.05ng/ml, corresponding to a Specific Activity of 9x108 IU/mg.
         
156 αα 17.3 kDa P06804
>98% E. coli -20oC


Physical Appearance

Sterile Filtered white lyophilized (freeze-dried) powder.

Formulation

Each mg contains 50mM Tris-HCL pH-8 and 20mM NaCL.

Reconstitution

Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.

Stability

Lyophilized product is very stable at -20 degrees. Reconstituted material should be aliquoted and frozen at -20. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.

Endotoxin Level

Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.

Protein Content determined by

UV spectroscopy at 280 nm. Analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.

Alternative Names

tumour necrosis factor, TNF

AA Sequence

MLRSSSQNSS DKPVAHVVAN HQVEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLVY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK VNLLSAVKSP CPKDTPEGAE LKPWYEPIYL GGVFQLEKGD QLSAEVNLPK YLDFAESGQV YFGVIAL

RESEARCH USE ONLY